Detailed information of TA system TA218221

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3902026..3902438
• Replicon: chromosome
• Accession: NZ_CP084205
• Organism: Escherichia coli strain EC2648-R1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LDB14_RS19025
• Protein ID: WP_000132601.1
• Coordinates: 3902097..3902438 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3902026..3902102 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB14_RS19015 (3898889)	3898889..3900478	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
LDB14_RS19020 (3900475)	3900475..3901869	+	1395	WP_072647344.1	type I restriction-modification system specificity subunit	-
- (3902026)	3902026..3902102	-	77	NuclAT_14	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_14	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_14	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_14	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_15	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_15	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_15	-	Antitoxin
- (3902026)	3902026..3902102	-	77	NuclAT_15	-	Antitoxin
LDB14_RS19025 (3902097)	3902097..3902438	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
LDB14_RS19030 (3902600)	3902600..3903979	+	1380	WP_072647343.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LDB14_RS19035 (3903979)	3903979..3905025	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T218221 WP_000132601.1 NZ_CP084205:3902097-3902438 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T218221 NZ_CP115701:740739-741224 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT218221 NZ_CP084205:c3902102-3902026 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin