Detailed information of TA system TA218219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3587586..3588280
• Replicon: chromosome
• Accession: NZ_CP084205
• Organism: Escherichia coli strain EC2648-R1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LDB14_RS17545
• Protein ID: WP_001263489.1
• Coordinates: 3587586..3587984 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LDB14_RS17550
• Protein ID: WP_000554758.1
• Coordinates: 3587987..3588280 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3583174)	3583174..3583254	-	81	NuclAT_12	-	-
- (3583174)	3583174..3583254	-	81	NuclAT_12	-	-
- (3583174)	3583174..3583254	-	81	NuclAT_12	-	-
- (3583174)	3583174..3583254	-	81	NuclAT_12	-	-
LDB14_RS17520 (3583850)	3583850..3584308	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LDB14_RS17525 (3584569)	3584569..3586026	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LDB14_RS17530 (3586083)	3586083..3586604	-	522	Protein_3446	peptide chain release factor H	-
LDB14_RS17535 (3586600)	3586600..3586806	-	207	Protein_3447	RtcB family protein	-
LDB14_RS17540 (3587124)	3587124..3587576	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LDB14_RS17545 (3587586)	3587586..3587984	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LDB14_RS17550 (3587987)	3587987..3588280	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LDB14_RS17555 (3588332)	3588332..3589387	-	1056	WP_001226164.1	DNA polymerase IV	-
LDB14_RS17560 (3589458)	3589458..3590243	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LDB14_RS17565 (3590215)	3590215..3591927	+	1713	Protein_3453	flagellar biosynthesis protein FlhA	-
LDB14_RS17570 (3592151)	3592151..3592648	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3574363..3588280	13917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T218219 WP_001263489.1 NZ_CP084205:c3587984-3587586 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T218219 NZ_CP115701:325944-326312 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT218219 WP_000554758.1 NZ_CP084205:c3588280-3587987 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT218219 NZ_CP115701:325726-325947 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3