Detailed information of TA system TA218191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 194973..195694
• Replicon: plasmid pCB1A
• Accession: NZ_CP084198
• Organism: Pantoea agglomerans strain CB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDO72_RS20290
• Protein ID: WP_143789442.1
• Coordinates: 194973..195284 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDO72_RS20295
• Protein ID: WP_031590724.1
• Coordinates: 195281..195694 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO72_RS20280 (LDO72_20305)	191676..192968	-	1293	WP_039389956.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
LDO72_RS20285 (LDO72_20310)	192979..194532	-	1554	WP_275103279.1	aspartate aminotransferase family protein	-
LDO72_RS20290 (LDO72_20315)	194973..195284	+	312	WP_143789442.1	type II toxin-antitoxin system HigB family toxin	Toxin
LDO72_RS20295 (LDO72_20320)	195281..195694	+	414	WP_031590724.1	helix-turn-helix domain-containing protein	Antitoxin
LDO72_RS20300 (LDO72_20325)	195810..197183	-	1374	WP_031590723.1	PTS ascorbate transporter subunit IIC	-
LDO72_RS20305 (LDO72_20330)	197223..197495	-	273	WP_003855084.1	PTS sugar transporter subunit IIB	-
LDO72_RS20310 (LDO72_20335)	197544..197993	-	450	WP_275103280.1	PTS sugar transporter subunit IIA	-
LDO72_RS20315 (LDO72_20340)	198570..199304	+	735	WP_275103281.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..569555	569555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12181.08 Da
• Isoelectric Point: 9.9086

>T218191 WP_143789442.1 NZ_CP084198:194973-195284 [Pantoea agglomerans]
MHIISRGPFREAAIIYPNHASALDAAYLVLRDENFATSDALKIRFQSLDRMKYREKWWVIDVGGNSLRIMFYADFDRGKI
FIKHIVTHAEYDKLVKKFRESKE

Nucleotide

• Length: 312 bp

>T218191 NZ_CP115672:3568313-3568612 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15420.44 Da
• Isoelectric Point: 4.9795

>AT218191 WP_031590724.1 NZ_CP084198:195281-195694 [Pantoea agglomerans]
MITEAIQATNDLVRIVPFLGGSTDKRDYEQALELVEYLVEHQPDSPLVEILSDKVARYENSAPEFAAFNARTDAMPRGVA
LLRVIMDQHGLTQSSFADEIGQRSYVSRILRGDRPLTDKHKARLAARFNLPFEAFAE

Nucleotide

• Length: 414 bp

>AT218191 NZ_CP115672:3568623-3568988 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA