Detailed information of TA system TA218190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 177252..177970
• Replicon: plasmid pCB1A
• Accession: NZ_CP084198
• Organism: Pantoea agglomerans strain CB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDO72_RS20220
• Protein ID: WP_099257039.1
• Coordinates: 177252..177563 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDO72_RS20225
• Protein ID: WP_275103273.1
• Coordinates: 177560..177970 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO72_RS20205 (LDO72_20230)	172533..173639	+	1107	WP_275103271.1	site-specific integrase	-
LDO72_RS20210 (LDO72_20235)	173658..174227	-	570	WP_275103272.1	lipid IV(A) palmitoyltransferase PagP	-
LDO72_RS20215 (LDO72_20240)	174672..176894	+	2223	WP_083069710.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
LDO72_RS20220 (LDO72_20245)	177252..177563	+	312	WP_099257039.1	type II toxin-antitoxin system HigB family toxin	Toxin
LDO72_RS20225 (LDO72_20250)	177560..177970	+	411	WP_275103273.1	helix-turn-helix domain-containing protein	Antitoxin
LDO72_RS20230 (LDO72_20255)	178085..178930	-	846	WP_275103274.1	VOC family protein	-
LDO72_RS20235 (LDO72_20260)	178951..180090	-	1140	WP_115388303.1	amidohydrolase	-
LDO72_RS20240 (LDO72_20265)	180093..181193	-	1101	WP_275103275.1	alkylhydroperoxidase domain protein	-
LDO72_RS20245 (LDO72_20270)	181190..182197	-	1008	WP_064702860.1	putative FMN-dependent luciferase-like monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..569555	569555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12196.11 Da
• Isoelectric Point: 9.8425

>T218190 WP_099257039.1 NZ_CP084198:177252-177563 [Pantoea agglomerans]
MHVISRVPFDEAARKYPNAAAALNLLYRHLRQGEFSGPDDLKALYASLDRMKYRAKWWVIDVSGNQLRVMFFADFERSKI
FIKHICTHAEYDKLVKKYREVTE

Nucleotide

• Length: 312 bp

>T218190 NZ_CP115672:c2726957-2726854 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15262.23 Da
• Isoelectric Point: 4.4192

>AT218190 WP_275103273.1 NZ_CP084198:177560-177970 [Pantoea agglomerans]
MNTEAIRLSNELAEAVPLLAGSSQRQDYEEALELVEYLIEHEPDNPLVDLICARIAHYEDKDPELAEFNARQSEISTGPG
ILRVLIEQHNLSLSDFENEIGKKSLVSRILSGERQLTVSHIRALCARFGLSPSLFF

Nucleotide

• Length: 411 bp

>AT218190 NZ_CP115672:2726849-2727050 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGCAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC