Detailed information of TA system TA218182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1776708..1777438
• Replicon: chromosome
• Accession: NZ_CP084197
• Organism: Pantoea agglomerans strain CB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LDO72_RS08340
• Protein ID: WP_143990299.1
• Coordinates: 1777121..1777438 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDO72_RS08335
• Protein ID: WP_275103004.1
• Coordinates: 1776708..1777040 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO72_RS08315 (LDO72_08305)	1772086..1772727	+	642	WP_003849160.1	protein phosphatase CheZ	-
LDO72_RS08320 (LDO72_08310)	1772916..1774067	+	1152	WP_031591448.1	flagellar biosynthesis protein FlhB	-
LDO72_RS08325 (LDO72_08315)	1774060..1776153	+	2094	WP_010670817.1	flagellar biosynthesis protein FlhA	-
LDO72_RS08330 (LDO72_08320)	1776153..1776545	+	393	WP_031591447.1	flagellar protein FlhE	-
LDO72_RS08335 (LDO72_08325)	1776708..1777040	-	333	WP_275103004.1	HigA family addiction module antitoxin	Antitoxin
LDO72_RS08340 (LDO72_08330)	1777121..1777438	-	318	WP_143990299.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDO72_RS08345 (LDO72_08335)	1777513..1778511	-	999	WP_109650567.1	aldo/keto reductase	-
LDO72_RS08350 (LDO72_08340)	1778721..1779656	-	936	WP_109650566.1	LysR family transcriptional regulator	-
LDO72_RS08355 (LDO72_08345)	1779744..1781474	-	1731	WP_010258588.1	arginine--tRNA ligase	-
LDO72_RS08360	1781685..1781807	-	123	WP_009089698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (8-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-99)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12370.17 Da
• Isoelectric Point: 9.8815

>T218182 WP_143990299.1 NZ_CP084197:c1777438-1777121 [Pantoea agglomerans]
VATLRNIESFRDDWLKDYFLYGKFSKRIPSSLSSALARKLDIINAAINYKDLRSPPGNRYEELNPPLEGYASIRVNEQYR
LIFKWIEGKAVDLYLDAHSYKMHKR

Nucleotide

• Length: 318 bp

>T218182 NZ_CP115670:2973867-2974346 [Corynebacterium sp. SCR221107]
GTGACCATCACTAACGAACAAGAGTGGGTTCAGCTACTCACGGATCTTGCGCTCAGGCTCAACCAAGAATGCGGAACGCG
TCAGAATGGCGCATCCCATGATGAGTACGCGGGATTCAGTTACGACGAAGGCGCTTTGTGTTCTGCCATCGCGGCCCCGT
TTCAAAGCGTTTTCGGCGCTCCGCGTTATGAATCGGTCTACGACCGCGCAGCCGCGCTCATGGCCCATATCGCAAAGGCC
CACGCCTTTAGGGACGGAAACAAGCGGACCGCTCTCAAGCTGTCCCTGGTGTATCTCACCATGAGGGGAATCAGGGTGGT
GCCTCCTAGCGCTGAGCTTGGTGCTCAGCTTGTTGAGAAGTGCGTGCTGGCCAACGGTAACCAGTTCGAACAGATGGTTG
TCGAGATCAGCACATTCCTCATCGCTTGGACAGTTCCAGACCAGATCGCGCGCGAGCATCGAAGGAATGTCCAAGGATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12617.46 Da
• Isoelectric Point: 5.1473

>AT218182 WP_275103004.1 NZ_CP084197:c1777040-1776708 [Pantoea agglomerans]
MNQATRKPTTVGDVLLYEYLEPTGLKILDLAEMLKVHRNTVSALVNNNRKLTPDMAFRLAAAFETSVEFWLNLQNSVDIW
EVMHDARAQEEISRVTPLKDFLTQKENQLA

Nucleotide

• Length: 333 bp

>AT218182 NZ_CP115670:2973297-2973863 [Corynebacterium sp. SCR221107]
ATGGCCGACATTCGCGACGTTGGGCAGTACATAGTTGAGCTCCGCGGCGGGACAGTTGATAAGCACAAGCTGGCCAAGCT
TTGTTTCTTCGCACAGGGGTGGCATTTGGCGTGGACTGGAAAGCCTTTGACGACAAGCCATTTCCAAGCGTGGATTTACG
GACCCGTGCCCACGGAGCTCGGGCGGTACAGCACTGTCGCCGAAGGATCTAAGGATGTGACGCGGATCGCCACGGGTGAC
TCTTCTCGTCTGAGCGACTACGAACGCGCGGTGGTTGCAAGCATTGTCGATTTCTACGGCGACATGGATTTTGCTGTCTT
GACGAAGCTCAGCCATGGCCGGTCGTGGGAAGAGGCACGCCAAGGCCTTCCCGAGAATGCTCCTTCGAGTGAGAATCTCA
GCGTCAGCACCATGCGGGAGGAATTCTCCCATCTGGTCCATGACAGCGGGGAGCTGCCGCAGGCCCCCACATTTAACGAG
GATCTTGATCTTGAGGCTGCCTTGGCAGCCTCCGAGCTCGTTGAGAACGATTGGATGGAAACCTTTCACCTCTTGGCCAC
CCGTTAA