Detailed information of TA system TA218153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 23394..24068
• Replicon: plasmid p1B17602
• Accession: NZ_CP084169
• Organism: Klebsiella pneumoniae strain B17602

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: LDH50_RS25110
• Protein ID: WP_032720638.1
• Coordinates: 23745..24068 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LDH50_RS25105
• Protein ID: WP_032720637.1
• Coordinates: 23394..23696 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH50_RS25065 (LDH50_25065)	18587..19159	+	573	WP_044266753.1	hypothetical protein	-
LDH50_RS25070 (LDH50_25070)	19190..19684	+	495	WP_044266755.1	hypothetical protein	-
LDH50_RS25075 (LDH50_25075)	19987..20907	+	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
LDH50_RS25080 (LDH50_25080)	20919..21731	+	813	WP_223203129.1	TSUP family transporter	-
LDH50_RS25085 (LDH50_25085)	21733..22305	+	573	WP_032720635.1	GNAT family N-acetyltransferase	-
LDH50_RS25090 (LDH50_25090)	22410..22793	+	384	WP_032720636.1	hypothetical protein	-
LDH50_RS25095 (LDH50_25095)	22790..22996	+	207	WP_153591439.1	hypothetical protein	-
LDH50_RS25100 (LDH50_25100)	23184..23351	+	168	WP_153591440.1	hypothetical protein	-
LDH50_RS25105 (LDH50_25105)	23394..23696	-	303	WP_032720637.1	NadS family protein	Antitoxin
LDH50_RS25110 (LDH50_25110)	23745..24068	-	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDH50_RS25115 (LDH50_25115)	24246..24651	-	406	Protein_28	DUF4113 domain-containing protein	-
LDH50_RS25120 (LDH50_25120)	24629..25056	+	428	Protein_29	DNA-binding protein	-
LDH50_RS25125 (LDH50_25125)	25125..25727	-	603	Protein_30	RNA-guided endonuclease TnpB family protein	-
LDH50_RS25130 (LDH50_25130)	26228..26551	-	324	WP_019725650.1	hypothetical protein	-
LDH50_RS25135 (LDH50_25135)	26624..27619	-	996	WP_019725651.1	hypothetical protein	-
LDH50_RS25140 (LDH50_25140)	27916..28566	-	651	WP_015065500.1	SOS response-associated peptidase	-
LDH50_RS25145 (LDH50_25145)	28613..29011	-	399	Protein_34	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	-	1..137142	137142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T218153 WP_032720638.1 NZ_CP084169:c24068-23745 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T218153 NZ_CP115661:328591-329067 [Pseudomonas putida]
TTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATATCTGGAT
CGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGA
TTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCTGACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCT
TTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGAATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGC
CAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGC
GAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT218153 WP_032720637.1 NZ_CP084169:c23696-23394 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT218153 NZ_CP115661:329109-329582 [Pseudomonas putida]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5