Detailed information of TA system TA218150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3700725..3701322
• Replicon: chromosome
• Accession: NZ_CP084168
• Organism: Klebsiella pneumoniae strain B17602

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LDH50_RS17830
• Protein ID: WP_004893639.1
• Coordinates: 3700725..3701042 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LDH50_RS17835
• Protein ID: WP_004142561.1
• Coordinates: 3701035..3701322 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH50_RS17815 (LDH50_17815)	3696429..3697856	-	1428	WP_004176980.1	MFS transporter	-
LDH50_RS17820 (LDH50_17820)	3697965..3698831	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LDH50_RS17825 (LDH50_17825)	3699497..3700540	+	1044	WP_004893645.1	DUF2157 domain-containing protein	-
LDH50_RS17830 (LDH50_17830)	3700725..3701042	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDH50_RS17835 (LDH50_17835)	3701035..3701322	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LDH50_RS17840 (LDH50_17840)	3701587..3702240	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LDH50_RS17845 (LDH50_17845)	3702360..3702728	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LDH50_RS17850 (LDH50_17850)	3702728..3703309	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
LDH50_RS17855 (LDH50_17855)	3703489..3704604	+	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
LDH50_RS17860 (LDH50_17860)	3704635..3704976	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LDH50_RS17865 (LDH50_17865)	3704994..3705242	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T218150 WP_004893639.1 NZ_CP084168:3700725-3701042 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T218150 NZ_CP115659:c3375490-3375188 [Kosakonia oryzendophytica]
ATGCCAAAGAAAATAAGTATTGCCTGTTTTCGGGATCCATGGCTTGAGGCATTTTTCCACCACACGGCGCAGCATCGTAA
AATTCCGGTGGACATTCATACCGTGTTGTCCCGAAAACTGGACATCATTAATGCAGCGACATCTCATCGCGATTTGCGCT
CGCCCCCCGGCAACCGTTATGAAGAGTTGTCGGGCAAATTGCATGGTTATTCCTCAATAAGAGTGAATAAGCAATATCGT
TTGATTTTCAAATGGGTTAACGGAAAAGCGGAAGATCTGTATCTGGATCCGCACATTTATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT218150 WP_004142561.1 NZ_CP084168:3701035-3701322 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT218150 NZ_CP115659:c3375165-3374824 [Kosakonia oryzendophytica]
ATGAAACCGGCCAGCCGAAAACCAACCACAGTGGGGGACATTCTGCTGTACGAATATCTGGAGCCGCTGGATCTCAGGAT
TAACGAGCTGGCTGAGATGCTGCATGTACATCGTAATTCTGTGAGTGCACTGGTTAACAACAATCGTCGCCTGACGACGG
ACATGGCGTTTCGTCTTGCCAGAGTCTTTGATACGTCTGTTGACTTCTGGATTAATCTCCAGGCTGCGGTGGATTTATGG
GATGTAGAGAATGATACGCGGGTGCAGGAAGAGCTGAGCCGCATTGAACCCGCCGCTGAATTTATCGCCCGGCGTGAAGC
AGGAACAAAAAAAGTCGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1