Detailed information of TA system TA218108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3740043..3740640
• Replicon: chromosome
• Accession: NZ_CP084103
• Organism: Klebsiella pneumoniae strain C11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: K8M04_RS18335
• Protein ID: WP_004893639.1
• Coordinates: 3740323..3740640 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: K8M04_RS18330
• Protein ID: WP_004142561.1
• Coordinates: 3740043..3740330 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8M04_RS18300 (K8M04_18265)	3736124..3736372	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
K8M04_RS18305 (K8M04_18270)	3736390..3736731	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
K8M04_RS18310 (K8M04_18275)	3736762..3737877	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
K8M04_RS18315 (K8M04_18280)	3738056..3738637	+	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
K8M04_RS18320 (K8M04_18285)	3738637..3739005	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
K8M04_RS18325 (K8M04_18290)	3739125..3739778	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
K8M04_RS18330 (K8M04_18295)	3740043..3740330	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
K8M04_RS18335 (K8M04_18300)	3740323..3740640	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8M04_RS18340 (K8M04_18305)	3740825..3741868	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
K8M04_RS18345 (K8M04_18310)	3742538..3743404	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
K8M04_RS18350 (K8M04_18315)	3743513..3744940	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T218108 WP_004893639.1 NZ_CP084103:c3740640-3740323 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T218108 NZ_CP115551:315844-316212 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT218108 WP_004142561.1 NZ_CP084103:c3740330-3740043 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT218108 NZ_CP115551:315626-315847 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1