Detailed information of TA system TA218092

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119348..119602
• Replicon: plasmid unnamed
• Accession: NZ_CP084097
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LCF29_RS25570
• Protein ID: WP_001312851.1
• Coordinates: 119348..119497 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	119541..119602 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS25535 (115678)	115678..115965	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LCF29_RS25540 (115962)	115962..116213	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LCF29_RS25545 (117176)	117176..118033	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
LCF29_RS25550 (118026)	118026..118508	-	483	WP_001273588.1	hypothetical protein	-
LCF29_RS25555 (118501)	118501..118548	-	48	WP_229471593.1	hypothetical protein	-
LCF29_RS25560 (118539)	118539..118790	+	252	WP_223195197.1	replication protein RepA	-
LCF29_RS25565 (118807)	118807..119064	-	258	WP_000083845.1	replication regulatory protein RepA	-
LCF29_RS25570 (119348)	119348..119497	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (119541)	119541..119602	+	62	NuclAT_1	-	Antitoxin
- (119541)	119541..119602	+	62	NuclAT_1	-	Antitoxin
- (119541)	119541..119602	+	62	NuclAT_1	-	Antitoxin
- (119541)	119541..119602	+	62	NuclAT_1	-	Antitoxin
LCF29_RS25575 (119858)	119858..119932	-	75	Protein_128	endonuclease	-
LCF29_RS25580 (120178)	120178..120390	-	213	WP_001299730.1	ANR family transcriptional regulator	-
LCF29_RS25585 (120526)	120526..121086	-	561	WP_000139315.1	fertility inhibition protein FinO	-
LCF29_RS25590 (121189)	121189..122049	-	861	WP_000704513.1	alpha/beta hydrolase	-
LCF29_RS25595 (122108)	122108..122854	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..148555	148555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T218092 WP_001312851.1 NZ_CP084097:c119497-119348 [Escherichia coli O78]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T218092 NZ_CP115461:1900264-1900518 [Coxiella burnetii]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT218092 NZ_CP084097:119541-119602 [Escherichia coli O78]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin