Detailed information of TA system TA218086

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12510..12936
• Replicon: plasmid unnamed
• Accession: NZ_CP084097
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCF29_RS25035
• Protein ID: WP_001372321.1
• Coordinates: 12510..12635 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	12712..12936 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS24995 (7880)	7880..8569	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
LCF29_RS25000 (8756)	8756..9139	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCF29_RS25005 (9460)	9460..10062	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
LCF29_RS25010 (10359)	10359..11180	-	822	WP_001234469.1	DUF932 domain-containing protein	-
LCF29_RS25015 (11301)	11301..11588	-	288	WP_000107535.1	hypothetical protein	-
LCF29_RS25020 (11613)	11613..11819	-	207	WP_000547939.1	hypothetical protein	-
LCF29_RS25025 (11889)	11889..12062	+	174	Protein_18	hypothetical protein	-
LCF29_RS25030 (12060)	12060..12290	-	231	WP_001426396.1	hypothetical protein	-
LCF29_RS25035 (12510)	12510..12635	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCF29_RS25040 (12577)	12577..12726	-	150	Protein_21	plasmid maintenance protein Mok	-
- (12712)	12712..12936	-	225	NuclAT_0	-	Antitoxin
- (12712)	12712..12936	-	225	NuclAT_0	-	Antitoxin
- (12712)	12712..12936	-	225	NuclAT_0	-	Antitoxin
- (12712)	12712..12936	-	225	NuclAT_0	-	Antitoxin
LCF29_RS25045 (12748)	12748..12936	+	189	WP_001299721.1	hypothetical protein	-
LCF29_RS25050 (12905)	12905..13667	-	763	Protein_23	plasmid SOS inhibition protein A	-
LCF29_RS25055 (13664)	13664..14098	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
LCF29_RS25060 (14153)	14153..16111	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
LCF29_RS25065 (16170)	16170..16403	-	234	WP_000006004.1	DUF905 domain-containing protein	-
LCF29_RS25070 (16459)	16459..16944	-	486	WP_042029316.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..148555	148555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T218086 WP_001372321.1 NZ_CP084097:c12635-12510 [Escherichia coli O78]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T218086 NZ_CP115456:1118423-1118938 [Sphingobium yanoikuyae]
ATGACCAGACGCCGTGTAACGCCGCCCGAGCGGTTGAACGCCGACCATTGCGTCGATGGGTTCGAGAATGGACGGCATGC
CTCGCTCGATCACTGGCTCGCAGAGCGGGCTCTCGCCAGCGAAGGCGCCTCGGCCCGCACCTATGTCGTGTGCGATGCCG
ACCGACCAGGCCATGTCGCGGGCTATTACACCATCACCACCGCCATGGAGGAGCGGGCCGCGCTCCCTACCGCCAGGTTG
CGGCGCGGGATGCCGGACAAGGTTCCGCTGCTCCTCATTGCCCGGCTTGCCGTGGACGTTGGCTTTCAGGGCCTCGGTCT
CGGCGCCGATCTGCTTGCCGATGCCCTGCGGCGATGTGCCGCTGCCTCGGAGATCGCTGGTGTCCGCGCGGTCATCGTGC
ATGCGATCGACGACGCGGCGGTCGGATTCTACGAGCGGCATGGGTTCATTGCCTCTCCACTCGGAGAACGCGTCCTGCTC
ATGCCCATCGAGGCGGTGCGAGCTCTGCAAGGCTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT218086 NZ_CP084097:c12936-12712 [Escherichia coli O78]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin