Detailed information of TA system TA218085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5008529..5009131
• Replicon: chromosome
• Accession: NZ_CP084096
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LCF29_RS23960
• Protein ID: WP_000897305.1
• Coordinates: 5008820..5009131 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCF29_RS23955
• Protein ID: WP_000356397.1
• Coordinates: 5008529..5008819 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS23930 (5004476)	5004476..5005378	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LCF29_RS23935 (5005375)	5005375..5006010	+	636	WP_000829031.1	formate dehydrogenase cytochrome b556 subunit	-
LCF29_RS23940 (5006007)	5006007..5006936	+	930	WP_000027691.1	formate dehydrogenase accessory protein FdhE	-
LCF29_RS23945 (5007266)	5007266..5007508	-	243	WP_001086385.1	protein YiiF	-
LCF29_RS23950 (5007727)	5007727..5007945	-	219	WP_001313440.1	CopG family transcriptional regulator	-
LCF29_RS23955 (5008529)	5008529..5008819	-	291	WP_000356397.1	NadS family protein	Antitoxin
LCF29_RS23960 (5008820)	5008820..5009131	-	312	WP_000897305.1	hypothetical protein	Toxin
LCF29_RS23965 (5009360)	5009360..5010268	+	909	WP_225343661.1	alpha/beta hydrolase	-
LCF29_RS23970 (5010436)	5010436..5011350	-	915	WP_191663692.1	transposase	-
LCF29_RS23975 (5011360)	5011360..5012250	-	891	Protein_4689	hypothetical protein	-
LCF29_RS23980 (5012661)	5012661..5013602	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
LCF29_RS23985 (5013647)	5013647..5014084	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T218085 WP_000897305.1 NZ_CP084096:c5009131-5008820 [Escherichia coli O78]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T218085 NZ_CP115456:c1099703-1099296 [Sphingobium yanoikuyae]
GTGATCGTAGACACCTCGGCGCTGGTCGCCATTCTTTATCGCGAGGAGGAGGCAGAGGATTTTGCCGATCTCATCCAGCG
CGCCGATATCTGCCGGATGAGTGTCGCGACCTATCTTGAACTCACCCTTGTCATCGAGAAGCAGTTGGGGCCTGAAGGCA
CGCGGCATGCGGAGAATTTCATCCGGCGCGCCGGCATCCAGATCGAGCCGGTCACCGTAGAGCAGGGCCTCATCGCCCGG
CAGGCCTTTTTCGATTTCGGCAAGGGGCGCCATTCGGCCGCGCTGAATTATGGCGACTGCTTTCCCTATGCGCTGGCGAA
AGCCGTCGACGAACCACTGCTCTTCAAGGGCAATGATTTCAGCCAGACCGACGTGGAAAGCGCCCTGCCGCGCGCCCGTC
CCCAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT218085 WP_000356397.1 NZ_CP084096:c5008819-5008529 [Escherichia coli O78]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT218085 NZ_CP115456:c1099954-1099700 [Sphingobium yanoikuyae]
ATGGGCGGTACACTCAATATCAAGGACCCCGAAGCGCACCGCCTGGCGCAGGCGATCGCGCAGGAGACCGGGGAGCCCAT
GACCCGTGTCGTGGTCGAGGCGCTTCGTGATCGGCTGTCCCGTATCGAACGCCAGCGCGAACGCGCCAGCGTGGGGGAAC
TGCTGGGCATCGCCGATCGTGCCGCTCGCCTCGTCGTCAAGCCCTACCCAAAGCATGGCGAGGAACTCTACGATGAGAAC
GGGCTTCCGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7