Detailed information of TA system TA218063

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2821507..2821878
• Replicon: chromosome
• Accession: NZ_CP084096
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: LCF29_RS13510
• Protein ID: WP_001317028.1
• Coordinates: 2821684..2821878 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2821507..2821685 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS13480 (2817259)	2817259..2817429	+	171	WP_001625136.1	protein YnaL	-
LCF29_RS13485 (2817462)	2817462..2818835	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
LCF29_RS13490 (2818964)	2818964..2819899	-	936	WP_001157403.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LCF29_RS13495 (2819951)	2819951..2821186	-	1236	WP_000040852.1	site-specific integrase	-
LCF29_RS13500 (2821188)	2821188..2821403	-	216	WP_000079604.1	excisionase XisR	-
- (2821507)	2821507..2821685	+	179	NuclAT_0	-	Antitoxin
- (2821507)	2821507..2821685	+	179	NuclAT_0	-	Antitoxin
- (2821507)	2821507..2821685	+	179	NuclAT_0	-	Antitoxin
- (2821507)	2821507..2821685	+	179	NuclAT_0	-	Antitoxin
LCF29_RS13505 (2821482)	2821482..2821691	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
LCF29_RS13510 (2821684)	2821684..2821878	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LCF29_RS13515 (2821935)	2821935..2822744	-	810	WP_000166319.1	recombination protein RecT	-
LCF29_RS13520 (2822737)	2822737..2825337	-	2601	WP_225343544.1	exodeoxyribonuclease VIII	-
LCF29_RS13525 (2825439)	2825439..2825714	-	276	WP_000632297.1	protein RacC	-
LCF29_RS13530 (2825789)	2825789..2825959	-	171	WP_001352098.1	YdaE family protein	-
LCF29_RS13535 (2825959)	2825959..2826180	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2801141..2864428	63287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T218063 WP_001317028.1 NZ_CP084096:c2821878-2821684 [Escherichia coli O78]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T218063 NZ_CP115447:c2906844-2906452 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT218063 NZ_CP084096:2821507-2821685 [Escherichia coli O78]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin