Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1525494..1526035 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name yafQ Uniprot ID U9XF38
Locus tag LCF29_RS07320 Protein ID WP_001695460.1
Coordinates 1525494..1525772 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LCF29_RS07325 Protein ID WP_000729704.1
Coordinates 1525775..1526035 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS07305 (1523177) 1523177..1523755 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LCF29_RS07310 (1523860) 1523860..1524627 + 768 WP_000333379.1 class II glutamine amidotransferase -
LCF29_RS07315 (1524598) 1524598..1525338 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LCF29_RS07320 (1525494) 1525494..1525772 - 279 WP_001695460.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LCF29_RS07325 (1525775) 1525775..1526035 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LCF29_RS07330 (1526245) 1526245..1526994 + 750 WP_001695461.1 C40 family peptidase -
LCF29_RS07335 (1527271) 1527271..1527768 + 498 WP_000006265.1 REP-associated tyrosine transposase RayT -
LCF29_RS07340 (1527984) 1527984..1529324 - 1341 Protein_1440 FHIPEP family type III secretion protein -
LCF29_RS07345 (1529296) 1529296..1530081 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T218061 WP_001695460.1 NZ_CP084096:c1525772-1525494 [Escherichia coli O78]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYIMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T218061 NZ_CP115447:c2886610-2886254 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT218061 WP_000729704.1 NZ_CP084096:c1526035-1525775 [Escherichia coli O78]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT218061 NZ_CP115447:c2886824-2886594 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XF38

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References