Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 678512..679311 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LCF29_RS03395 Protein ID WP_000347273.1
Coordinates 678512..678976 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LCF29_RS03400 Protein ID WP_001307405.1
Coordinates 678976..679311 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS03365 (673513) 673513..673947 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LCF29_RS03370 (673965) 673965..674843 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LCF29_RS03375 (674833) 674833..675612 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LCF29_RS03380 (675623) 675623..676096 - 474 WP_001626031.1 PTS N-acetylgalactosamine transporter subunit IIB -
LCF29_RS03385 (676119) 676119..677399 - 1281 WP_000681939.1 tagatose-bisphosphate aldolase subunit KbaZ -
LCF29_RS03390 (677648) 677648..678457 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LCF29_RS03395 (678512) 678512..678976 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LCF29_RS03400 (678976) 678976..679311 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LCF29_RS03405 (679460) 679460..681031 - 1572 WP_001273763.1 galactarate dehydratase -
LCF29_RS03410 (681406) 681406..682740 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LCF29_RS03415 (682756) 682756..683526 + 771 WP_001058233.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T218056 WP_000347273.1 NZ_CP084096:c678976-678512 [Escherichia coli O78]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T218056 NZ_CP115447:2644698-2645075 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT218056 WP_001307405.1 NZ_CP084096:c679311-678976 [Escherichia coli O78]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT218056 NZ_CP115447:2644444-2644701 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References