Detailed information of TA system TA218048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3847407..3848101
• Replicon: chromosome
• Accession: NZ_CP084055
• Organism: Escherichia coli strain X14-3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: LCR13_RS18805
• Protein ID: WP_001263493.1
• Coordinates: 3847703..3848101 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LCR13_RS18800
• Protein ID: WP_000554757.1
• Coordinates: 3847407..3847700 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCR13_RS18780 (3843039)	3843039..3843536	+	498	WP_254825356.1	REP-associated tyrosine transposase RayT	-
LCR13_RS18785 (3843760)	3843760..3845472	-	1713	Protein_3671	flagellar biosynthesis protein FlhA	-
LCR13_RS18790 (3845444)	3845444..3846229	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
LCR13_RS18795 (3846300)	3846300..3847355	+	1056	WP_001226164.1	DNA polymerase IV	-
LCR13_RS18800 (3847407)	3847407..3847700	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LCR13_RS18805 (3847703)	3847703..3848101	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LCR13_RS18810 (3848111)	3848111..3848563	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
LCR13_RS18815 (3848809)	3848809..3849012	+	204	Protein_3677	RtcB family protein	-
LCR13_RS18820 (3849011)	3849011..3849532	+	522	Protein_3678	peptide chain release factor H	-
LCR13_RS18825 (3849589)	3849589..3851046	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
LCR13_RS18830 (3851307)	3851307..3851765	+	459	WP_254825357.1	xanthine phosphoribosyltransferase	-
- (3852361)	3852361..3852441	+	81	NuclAT_11	-	-
- (3852361)	3852361..3852441	+	81	NuclAT_11	-	-
- (3852361)	3852361..3852441	+	81	NuclAT_11	-	-
- (3852361)	3852361..3852441	+	81	NuclAT_11	-	-
LCR13_RS18835 (3851857)	3851857..3853101	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3833147..3860113	26966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T218048 WP_001263493.1 NZ_CP084055:3847703-3848101 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T218048 NZ_CP115447:c2004493-2004149 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT218048 WP_000554757.1 NZ_CP084055:3847407-3847700 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT218048 NZ_CP115447:c2004735-2004487 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6