Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3947050..3947647 Replicon chromosome
Accession NZ_CP084044
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWJ12_RS19430 Protein ID WP_004142563.1
Coordinates 3947330..3947647 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWJ12_RS19425 Protein ID WP_004142561.1
Coordinates 3947050..3947337 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWJ12_RS19395 (3943145) 3943145..3943393 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWJ12_RS19400 (3943411) 3943411..3943752 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWJ12_RS19405 (3943783) 3943783..3944898 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KWJ12_RS19410 (3945078) 3945078..3945662 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KWJ12_RS19415 (3945659) 3945659..3946027 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KWJ12_RS19420 (3946147) 3946147..3946785 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KWJ12_RS19425 (3947050) 3947050..3947337 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWJ12_RS19430 (3947330) 3947330..3947647 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWJ12_RS19435 (3947832) 3947832..3948875 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KWJ12_RS19440 (3949545) 3949545..3950411 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KWJ12_RS19445 (3950520) 3950520..3951947 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T218020 WP_004142563.1 NZ_CP084044:c3947647-3947330 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T218020 NZ_CP115307:2331867-2332604 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCAAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT218020 WP_004142561.1 NZ_CP084044:c3947337-3947050 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT218020 NZ_CP115307:2332717-2332845 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAAATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References