Detailed information of TA system TA217994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 510795..511297
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A7W2E5L4
• Locus tag: LCF41_RS02350
• Protein ID: WP_039282578.1
• Coordinates: 510795..511049 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LCF41_RS02355
• Protein ID: WP_225086722.1
• Coordinates: 511046..511297 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS02330	505950..507212	-	1263	WP_225086718.1	L-rhamnose isomerase	-
LCF41_RS02335	507209..508699	-	1491	WP_225086719.1	rhamnulokinase	-
LCF41_RS02340	509035..509874	+	840	WP_225086720.1	HTH-type transcriptional activator RhaS	-
LCF41_RS02345	509899..510753	+	855	WP_225086721.1	helix-turn-helix domain-containing protein	-
LCF41_RS02350	510795..511049	-	255	WP_039282578.1	Txe/YoeB family addiction module toxin	Toxin
LCF41_RS02355	511046..511297	-	252	WP_225086722.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LCF41_RS02360	511373..513076	-	1704	WP_225086723.1	ABC transporter ATP-binding protein	-
LCF41_RS02365	513073..513939	-	867	WP_225086724.1	ABC transporter permease	-
LCF41_RS02370	513932..514891	-	960	WP_012773138.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10229.79 Da
• Isoelectric Point: 8.0353

>T217994 WP_039282578.1 NZ_CP084032:c511049-510795 [Pectobacterium colocasium]
VKLIWSEEAWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T217994 NZ_CP115158:470722-471072 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9390.68 Da
• Isoelectric Point: 4.8223

>AT217994 WP_225086722.1 NZ_CP084032:c511297-511046 [Pectobacterium colocasium]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGHGEERD
IIE

Nucleotide

• Length: 252 bp

>AT217994 NZ_CP115158:470435-470716 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A7W2E5L4