Detailed information of TA system TA217975

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3848956..3849368
• Replicon: chromosome
• Accession: NZ_CP084026
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LC440_RS18915
• Protein ID: WP_000132601.1
• Coordinates: 3849027..3849368 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3848956..3849032 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC440_RS18905 (3845819)	3845819..3847408	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
LC440_RS18910 (3847405)	3847405..3848799	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (3848956)	3848956..3849032	-	77	NuclAT_14	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_14	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_14	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_14	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_15	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_15	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_15	-	Antitoxin
- (3848956)	3848956..3849032	-	77	NuclAT_15	-	Antitoxin
LC440_RS18915 (3849027)	3849027..3849368	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
LC440_RS18920 (3849530)	3849530..3850909	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LC440_RS18925 (3850909)	3850909..3851955	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T217975 WP_000132601.1 NZ_CP084026:3849027-3849368 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T217975 NZ_CP114896:c4000512-4000255 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT217975 NZ_CP084026:c3849032-3848956 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin