Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3540713..3541254 Replicon chromosome
Accession NZ_CP084026
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LC440_RS17475 Protein ID WP_000615983.1
Coordinates 3540976..3541254 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LC440_RS17470 Protein ID WP_000729703.1
Coordinates 3540713..3540973 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC440_RS17450 (3536388) 3536388..3537173 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LC440_RS17455 (3537145) 3537145..3538857 + 1713 Protein_3414 flagellar biosynthesis protein FlhA -
LC440_RS17460 (3539081) 3539081..3539578 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LC440_RS17465 (3539754) 3539754..3540503 - 750 WP_000056849.1 C40 family peptidase -
LC440_RS17470 (3540713) 3540713..3540973 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LC440_RS17475 (3540976) 3540976..3541254 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LC440_RS17480 (3541410) 3541410..3542150 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LC440_RS17485 (3542121) 3542121..3542888 - 768 WP_000333380.1 class II glutamine amidotransferase -
LC440_RS17490 (3543094) 3543094..3543672 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3504752..3565601 60849


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T217974 WP_000615983.1 NZ_CP084026:3540976-3541254 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T217974 NZ_CP114896:3913705-3914055 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT217974 WP_000729703.1 NZ_CP084026:3540713-3540973 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT217974 NZ_CP114896:3913460-3913711 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References