Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3534516..3535210 Replicon chromosome
Accession NZ_CP084026
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LC440_RS17435 Protein ID WP_001263489.1
Coordinates 3534516..3534914 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LC440_RS17440 Protein ID WP_000554758.1
Coordinates 3534917..3535210 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3530104) 3530104..3530184 - 81 NuclAT_12 - -
- (3530104) 3530104..3530184 - 81 NuclAT_12 - -
- (3530104) 3530104..3530184 - 81 NuclAT_12 - -
- (3530104) 3530104..3530184 - 81 NuclAT_12 - -
LC440_RS17410 (3530780) 3530780..3531238 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LC440_RS17415 (3531499) 3531499..3532956 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LC440_RS17420 (3533013) 3533013..3533534 - 522 Protein_3407 peptide chain release factor H -
LC440_RS17425 (3533530) 3533530..3533736 - 207 Protein_3408 RtcB family protein -
LC440_RS17430 (3534054) 3534054..3534506 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LC440_RS17435 (3534516) 3534516..3534914 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LC440_RS17440 (3534917) 3534917..3535210 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LC440_RS17445 (3535262) 3535262..3536317 - 1056 WP_001226164.1 DNA polymerase IV -
LC440_RS17450 (3536388) 3536388..3537173 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LC440_RS17455 (3537145) 3537145..3538857 + 1713 Protein_3414 flagellar biosynthesis protein FlhA -
LC440_RS17460 (3539081) 3539081..3539578 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3504752..3565601 60849


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T217973 WP_001263489.1 NZ_CP084026:c3534914-3534516 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T217973 NZ_CP114896:3817921-3818094 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT217973 WP_000554758.1 NZ_CP084026:c3535210-3534917 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT217973 NZ_CP114896:3817597-3817893 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References