Detailed information of TA system TA217973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3534516..3535210
• Replicon: chromosome
• Accession: NZ_CP084026
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LC440_RS17435
• Protein ID: WP_001263489.1
• Coordinates: 3534516..3534914 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LC440_RS17440
• Protein ID: WP_000554758.1
• Coordinates: 3534917..3535210 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3530104)	3530104..3530184	-	81	NuclAT_12	-	-
- (3530104)	3530104..3530184	-	81	NuclAT_12	-	-
- (3530104)	3530104..3530184	-	81	NuclAT_12	-	-
- (3530104)	3530104..3530184	-	81	NuclAT_12	-	-
LC440_RS17410 (3530780)	3530780..3531238	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LC440_RS17415 (3531499)	3531499..3532956	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LC440_RS17420 (3533013)	3533013..3533534	-	522	Protein_3407	peptide chain release factor H	-
LC440_RS17425 (3533530)	3533530..3533736	-	207	Protein_3408	RtcB family protein	-
LC440_RS17430 (3534054)	3534054..3534506	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LC440_RS17435 (3534516)	3534516..3534914	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LC440_RS17440 (3534917)	3534917..3535210	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LC440_RS17445 (3535262)	3535262..3536317	-	1056	WP_001226164.1	DNA polymerase IV	-
LC440_RS17450 (3536388)	3536388..3537173	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LC440_RS17455 (3537145)	3537145..3538857	+	1713	Protein_3414	flagellar biosynthesis protein FlhA	-
LC440_RS17460 (3539081)	3539081..3539578	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3504752..3565601	60849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T217973 WP_001263489.1 NZ_CP084026:c3534914-3534516 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T217973 NZ_CP114896:3817921-3818094 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT217973 WP_000554758.1 NZ_CP084026:c3535210-3534917 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT217973 NZ_CP114896:3817597-3817893 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3