Detailed information of TA system TA021792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 10973..11555
• Replicon: plasmid pPRO2
• Accession: NC_008608
• Organism: Pelobacter propionicus DSM 2379

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PPRO_RS20540
• Protein ID: WP_011733943.1
• Coordinates: 11277..11555 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: PPRO_RS19065
• Protein ID: WP_041533075.1
• Coordinates: 10973..11254 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPRO_RS19040	6542..7015	-	474	WP_011733937.1	cytochrome c	-
PPRO_RS19045	7044..7754	-	711	WP_011733938.1	ABC transporter ATP-binding protein	-
PPRO_RS19050	7756..8961	-	1206	WP_011733939.1	ABC transporter permease	-
PPRO_RS19055	8958..10109	-	1152	WP_011733940.1	efflux RND transporter periplasmic adaptor subunit	-
PPRO_RS19060	10106..10714	-	609	WP_011733941.1	TetR/AcrR family transcriptional regulator	-
PPRO_RS19065	10973..11254	-	282	WP_041533075.1	HigA family addiction module antidote protein	Antitoxin
PPRO_RS20540	11277..11555	-	279	WP_011733943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPRO_RS20545	11895..12422	-	528	WP_011733944.1	GNAT family N-acetyltransferase	-
PPRO_RS19075	12419..12697	-	279	WP_011733945.1	DUF1778 domain-containing protein	-
PPRO_RS19085	13713..14495	+	783	WP_157040166.1	pirin	-
PPRO_RS19090	14980..15234	+	255	WP_011733948.1	ribbon-helix-helix domain-containing protein	-
PPRO_RS19095	15218..15499	+	282	WP_011733949.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PPRO_RS19100	15731..16123	-	393	WP_157040165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30722	30722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10610.14 Da
• Isoelectric Point: 8.9269

>T21792 WP_011733943.1 NC_008608:c11555-11277 [Pelobacter propionicus DSM 2379]
MIKSFNHKGLERYYESGNKAGIQAQYEKRIRLILGRLNASCEPKDMNLPGLRLHALQGALSDFWAVDVSGNWRIIFRFEG
PNVFDVNLVDYH

Nucleotide

• Length: 279 bp

>T21792 NC_008608:c11555-11277 [Pelobacter propionicus DSM 2379]
ATGATTAAATCCTTTAATCACAAAGGCCTGGAGCGCTACTACGAAAGCGGCAACAAAGCCGGCATCCAGGCGCAGTATGA
AAAACGCATTCGCCTTATTTTGGGCCGGCTCAACGCCTCTTGTGAACCAAAAGACATGAATCTCCCCGGCCTTCGTTTGC
ATGCCCTCCAGGGCGCACTGAGTGACTTCTGGGCTGTGGACGTCAGTGGCAATTGGAGAATTATTTTCAGGTTCGAAGGG
CCAAACGTTTTTGATGTCAACCTCGTCGATTATCATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10305.95 Da
• Isoelectric Point: 6.4581

>AT21792 WP_041533075.1 NC_008608:c11254-10973 [Pelobacter propionicus DSM 2379]
MFNPPHPGEIIRELCLEPLGVTVTEAAKALGVSRKALSELLNGHSRVSPEMAVRLSIALETTADSWLIQQAQYDLWLAEQ
KRDTLQVRILKAA

Nucleotide

• Length: 282 bp

>AT21792 NC_008608:c11254-10973 [Pelobacter propionicus DSM 2379]
ATGTTCAATCCTCCCCATCCGGGAGAAATCATCAGAGAATTGTGCCTGGAACCGCTCGGCGTCACCGTCACGGAGGCCGC
TAAAGCGCTGGGAGTCTCCCGCAAAGCGTTGTCGGAACTTTTGAACGGCCACTCCAGGGTCAGCCCCGAGATGGCCGTGC
GTCTTTCGATTGCGCTGGAAACGACCGCTGACAGCTGGCTGATCCAGCAGGCGCAATACGATCTCTGGCTCGCGGAGCAA
AAAAGGGATACCTTACAGGTAAGGATTTTAAAGGCTGCCTAG