Detailed information of TA system TA217907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 159792..160519
• Replicon: plasmid pKP2648-VirV
• Accession: NZ_CP083992
• Organism: Escherichia coli strain EC2648-Vir2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LCH23_RS23435
• Protein ID: WP_011251285.1
• Coordinates: 160208..160519 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCH23_RS23430
• Protein ID: WP_011251286.1
• Coordinates: 159792..160211 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH23_RS23400 (LCH23_23410)	154960..155430	-	471	WP_048333570.1	hypothetical protein	-
LCH23_RS23405 (LCH23_23415)	155743..156378	-	636	WP_223171879.1	hypothetical protein	-
LCH23_RS23410 (LCH23_23420)	156797..157417	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LCH23_RS23415 (LCH23_23425)	157438..158226	+	789	WP_040217257.1	hypothetical protein	-
LCH23_RS23420 (LCH23_23430)	158240..158605	+	366	WP_048333448.1	hypothetical protein	-
LCH23_RS23425 (LCH23_23435)	158677..159645	-	969	WP_074428168.1	IS5 family transposase	-
LCH23_RS23430 (LCH23_23440)	159792..160211	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LCH23_RS23435 (LCH23_23445)	160208..160519	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LCH23_RS23440 (LCH23_23450)	160724..161161	-	438	Protein_169	DDE-type integrase/transposase/recombinase	-
LCH23_RS23445 (LCH23_23455)	161296..161993	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
LCH23_RS23450 (LCH23_23460)	161995..162444	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LCH23_RS23455 (LCH23_23465)	162461..162772	+	312	WP_011251282.1	hypothetical protein	-
LCH23_RS23460 (LCH23_23470)	162786..163127	+	342	WP_011251281.1	hypothetical protein	-
LCH23_RS23465 (LCH23_23475)	163187..164143	+	957	WP_011251280.1	DsbA family protein	-
LCH23_RS23470 (LCH23_23480)	164568..165008	-	441	WP_011251275.1	hypothetical protein	-
LCH23_RS23475 (LCH23_23485)	165014..165508	-	495	WP_011251274.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA	1..222599	222599
	-	inside	IScluster/Tn	-	-	143021..172547	29526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T217907 WP_011251285.1 NZ_CP083992:c160519-160208 [Escherichia coli]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T217907 NZ_CP114894:c943716-942394 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT217907 WP_011251286.1 NZ_CP083992:c160211-159792 [Escherichia coli]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT217907 NZ_CP114894:c943982-943716 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1