Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3594768..3595309 Replicon chromosome
Accession NZ_CP083990
Organism Escherichia coli strain EC2648-Vir2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LCH23_RS17590 Protein ID WP_000615983.1
Coordinates 3595031..3595309 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LCH23_RS17585 Protein ID WP_000729703.1
Coordinates 3594768..3595028 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH23_RS17565 (3590443) 3590443..3591228 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LCH23_RS17570 (3591200) 3591200..3592912 + 1713 Protein_3455 flagellar biosynthesis protein FlhA -
LCH23_RS17575 (3593136) 3593136..3593633 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LCH23_RS17580 (3593809) 3593809..3594558 - 750 WP_000056849.1 C40 family peptidase -
LCH23_RS17585 (3594768) 3594768..3595028 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LCH23_RS17590 (3595031) 3595031..3595309 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LCH23_RS17595 (3595465) 3595465..3596205 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LCH23_RS17600 (3596176) 3596176..3596943 - 768 WP_000333380.1 class II glutamine amidotransferase -
LCH23_RS17605 (3597149) 3597149..3597727 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T217900 WP_000615983.1 NZ_CP083990:3595031-3595309 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T217900 NZ_CP114893:3894663-3894770 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT217900 WP_000729703.1 NZ_CP083990:3594768-3595028 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT217900 NZ_CP114893:c3894616-3894550 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References