Detailed information of TA system TA217899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3588571..3589265
• Replicon: chromosome
• Accession: NZ_CP083990
• Organism: Escherichia coli strain EC2648-Vir2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LCH23_RS17550
• Protein ID: WP_001263489.1
• Coordinates: 3588571..3588969 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LCH23_RS17555
• Protein ID: WP_000554758.1
• Coordinates: 3588972..3589265 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3584159)	3584159..3584239	-	81	NuclAT_12	-	-
- (3584159)	3584159..3584239	-	81	NuclAT_12	-	-
- (3584159)	3584159..3584239	-	81	NuclAT_12	-	-
- (3584159)	3584159..3584239	-	81	NuclAT_12	-	-
LCH23_RS17525 (3584835)	3584835..3585293	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LCH23_RS17530 (3585554)	3585554..3587011	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LCH23_RS17535 (3587068)	3587068..3587589	-	522	Protein_3448	peptide chain release factor H	-
LCH23_RS17540 (3587585)	3587585..3587791	-	207	Protein_3449	RtcB family protein	-
LCH23_RS17545 (3588109)	3588109..3588561	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LCH23_RS17550 (3588571)	3588571..3588969	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LCH23_RS17555 (3588972)	3588972..3589265	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LCH23_RS17560 (3589317)	3589317..3590372	-	1056	WP_001226164.1	DNA polymerase IV	-
LCH23_RS17565 (3590443)	3590443..3591228	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LCH23_RS17570 (3591200)	3591200..3592912	+	1713	Protein_3455	flagellar biosynthesis protein FlhA	-
LCH23_RS17575 (3593136)	3593136..3593633	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T217899 WP_001263489.1 NZ_CP083990:c3588969-3588571 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T217899 NZ_CP114893:c3606566-3606390 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT217899 WP_000554758.1 NZ_CP083990:c3589265-3588972 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT217899 NZ_CP114893:c3606344-3605928 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3