Detailed information of TA system TA217879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 717224..717917
• Replicon: chromosome
• Accession: NZ_CP083990
• Organism: Escherichia coli strain EC2648-Vir2

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LCH23_RS03495
• Protein ID: WP_000415584.1
• Coordinates: 717224..717520 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LCH23_RS03500
• Protein ID: WP_000650107.1
• Coordinates: 717522..717917 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH23_RS03460 (712312)	712312..712626	-	315	WP_000958598.1	putative quinol monooxygenase	-
LCH23_RS03465 (712657)	712657..713238	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LCH23_RS03470 (713557)	713557..713889	+	333	WP_000917684.1	DUF2645 family protein	-
LCH23_RS03475 (713935)	713935..715284	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LCH23_RS03480 (715281)	715281..715940	-	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
LCH23_RS03485 (716092)	716092..716484	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LCH23_RS03490 (716537)	716537..717019	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LCH23_RS03495 (717224)	717224..717520	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LCH23_RS03500 (717522)	717522..717917	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LCH23_RS03505 (718050)	718050..719657	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
LCH23_RS03510 (719795)	719795..722053	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T217879 WP_000415584.1 NZ_CP083990:717224-717520 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T217879 NZ_CP114893:276151-276492 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT217879 WP_000650107.1 NZ_CP083990:717522-717917 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT217879 NZ_CP114893:275813-276130 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65