Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2507991..2508631 Replicon chromosome
Accession NZ_CP083950
Organism Mesorhizobium sp. B4-1-4

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag FJW03_RS12180 Protein ID WP_140765174.1
Coordinates 2507991..2508287 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FJW03_RS12185 Protein ID WP_140765172.1
Coordinates 2508296..2508631 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJW03_RS12170 (FJW03_12170) 2505873..2507171 + 1299 WP_140765178.1 DUF3419 family protein -
FJW03_RS12175 (FJW03_12175) 2507168..2507833 + 666 WP_140765176.1 class I SAM-dependent methyltransferase -
FJW03_RS12180 (FJW03_12180) 2507991..2508287 + 297 WP_140765174.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FJW03_RS12185 (FJW03_12185) 2508296..2508631 + 336 WP_140765172.1 HigA family addiction module antitoxin Antitoxin
FJW03_RS12190 (FJW03_12190) 2508628..2510007 - 1380 WP_140765170.1 serine hydrolase -
FJW03_RS12195 (FJW03_12195) 2510293..2511084 + 792 WP_140765168.1 GH25 family lysozyme -
FJW03_RS12200 (FJW03_12200) 2511328..2512506 + 1179 WP_140765166.1 lytic murein transglycosylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11660.18 Da        Isoelectric Point: 6.2276

>T217840 WP_140765174.1 NZ_CP083950:2507991-2508287 [Mesorhizobium sp. B4-1-4]
VIVRFRDEWLRAFFVEDIPSRNIPPDLESRLFRKLQMIDDAMTDQDLGVPPSNHFEKLRGNLDGFHSIRVNKQWRLIFRW
DGGRGEAADIYLDDHSYK

Download         Length: 297 bp

>T217840 NZ_CP114891:1775802-1777124 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12336.26 Da        Isoelectric Point: 10.9269

>AT217840 WP_140765172.1 NZ_CP083950:2508296-2508631 [Mesorhizobium sp. B4-1-4]
MLMTTRKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
QVMNSPVERARVDRAKPLATAAQMTRQSGAV

Download         Length: 336 bp

>AT217840 NZ_CP114891:1775536-1775802 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References