Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3962162..3962759 Replicon chromosome
Accession NZ_CP083932
Organism Klebsiella pneumoniae strain BA4946

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LCH45_RS19270 Protein ID WP_004142563.1
Coordinates 3962442..3962759 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LCH45_RS19265 Protein ID WP_004142561.1
Coordinates 3962162..3962449 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH45_RS19235 (3958242) 3958242..3958490 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LCH45_RS19240 (3958508) 3958508..3958849 - 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
LCH45_RS19245 (3958880) 3958880..3959995 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
LCH45_RS19250 (3960175) 3960175..3960756 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
LCH45_RS19255 (3960756) 3960756..3961124 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LCH45_RS19260 (3961244) 3961244..3961894 + 651 Protein_3779 oxygen-insensitive NAD(P)H nitroreductase -
LCH45_RS19265 (3962162) 3962162..3962449 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LCH45_RS19270 (3962442) 3962442..3962759 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCH45_RS19275 (3962944) 3962944..3963987 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
LCH45_RS19280 (3964654) 3964654..3965520 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
LCH45_RS19285 (3965629) 3965629..3967056 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T217819 WP_004142563.1 NZ_CP083932:c3962759-3962442 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T217819 NZ_CP114886:c5135968-5135642 [Endozoicomonas sp. GU-1]
ATGGAATTTGTAGAAGCCAGCATATTTACCAAAATCATCACCCAGCTCATGTCTGATGATGATTATCGAGCCATGCAGGA
AGCCATGATCGCACAGCCTGATATAGGCAGTGTTATCAGGGGTACTGGGGGGCTACGGAAGTTCCGCTGGAAGTTGGGTG
ACTCGGGCAAGCGAGGAGGTGTGCGAACCATCTATTACTGGCAGGTACATGAAGAGACCTTCTACATGCTGTATGCCTAT
ACCAAAAACCGGCAAAAAGACTTAACAAGTGTTGAGAAAAAAGTACTTTCAGAACTGGCGAGGGAGTTTTGCAATGAGCG
ACCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT217819 WP_004142561.1 NZ_CP083932:c3962449-3962162 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT217819 NZ_CP114886:c5135655-5135353 [Endozoicomonas sp. GU-1]
ATGAGCGACCATGAATTTAACTTTGATGAATTGGTGGAGAGCGTCAGGGAAGCGATTGACATTAATAAAGGCATTAAGAA
ACCTTCACGGTCGTTCAAATATGAAGAGATTGATGTCTTTGCTATCAGGAAAGCAACACACCTTTCGCAGGAGAAGTTTG
CGGCGCTTATTGGGGTGAGCATCAAGACAGTGCAGAGTTGGGAACAAAAGGTGAGAAAACCGCTCGGGCCAGCCAGATCG
CTACTGATTATGTTTCGCAACAACCCTGACCAGGCAATGGCTCTACTTCATCAATCAAGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References