Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yefM-yoeB (relBE)/Txe-RelB |
| Location | 198000..198535 | Replicon | plasmid pV1164-1 |
| Accession | NZ_CP083921 | ||
| Organism | Enterococcus faecium isolate V1164 | ||
Toxin (Protein)
| Gene name | yoeB | Uniprot ID | R2PQC0 |
| Locus tag | LCG90_RS14310 | Protein ID | WP_002314366.1 |
| Coordinates | 198000..198272 (-) | Length | 91 a.a. |
Antitoxin (Protein)
| Gene name | yefM | Uniprot ID | Q3Y220 |
| Locus tag | LCG90_RS14315 | Protein ID | WP_002297422.1 |
| Coordinates | 198272..198535 (-) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCG90_RS14275 (LCG90_14290) | 193986..194510 | + | 525 | Protein_214 | transposase | - |
| LCG90_RS14280 (LCG90_14295) | 194768..195238 | - | 471 | Protein_215 | sigma 54-interacting transcriptional regulator | - |
| LCG90_RS14285 (LCG90_14300) | 195279..195927 | + | 649 | Protein_216 | IS6 family transposase | - |
| LCG90_RS14290 (LCG90_14305) | 195986..196174 | - | 189 | Protein_217 | hypothetical protein | - |
| LCG90_RS14295 (LCG90_14310) | 196175..197032 | - | 858 | WP_002292680.1 | ParA family protein | - |
| LCG90_RS14300 (LCG90_14315) | 197317..197604 | - | 288 | WP_002300557.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LCG90_RS14305 (LCG90_14320) | 197594..197923 | - | 330 | WP_002292678.1 | AbrB family transcriptional regulator | - |
| LCG90_RS14310 (LCG90_14325) | 198000..198272 | - | 273 | WP_002314366.1 | Txe/YoeB family addiction module toxin | Toxin |
| LCG90_RS14315 (LCG90_14330) | 198272..198535 | - | 264 | WP_002297422.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| LCG90_RS14320 (LCG90_14335) | 198769..199677 | + | 909 | Protein_223 | IS982 family transposase | - |
| LCG90_RS14325 (LCG90_14340) | 199680..200855 | - | 1176 | WP_002336205.1 | MFS transporter | - |
| LCG90_RS14330 (LCG90_14345) | 200858..203107 | - | 2250 | WP_002314387.1 | alpha-galactosidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') | - | 1..213279 | 213279 | |
| - | inside | IScluster/Tn | - | - | 189024..211027 | 22003 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 91 a.a. Molecular weight: 10780.24 Da Isoelectric Point: 9.9724
>T217801 WP_002314366.1 NZ_CP083921:c198272-198000 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ
Download Length: 273 bp
>T217801 NZ_CP114853:4245187-4245564 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA
Antitoxin
Download Length: 88 a.a. Molecular weight: 9959.22 Da Isoelectric Point: 4.5370
>AT217801 WP_002297422.1 NZ_CP083921:c198535-198272 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL
Download Length: 264 bp
>AT217801 NZ_CP114853:4244777-4245136 [Klebsiella pneumoniae]
TTGTCGAATAAAACACCGATAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGGCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
CGGTTTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA
TTGTCGAATAAAACACCGATAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGGCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
CGGTTTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F704 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F006 |