Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 198005..198540 Replicon plasmid pV1225-1
Accession NZ_CP083913
Organism Enterococcus faecium isolate V1225

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag LCG89_RS14395 Protein ID WP_002314366.1
Coordinates 198005..198277 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag LCG89_RS14400 Protein ID WP_002297422.1
Coordinates 198277..198540 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCG89_RS14360 (LCG89_14360) 193992..194516 + 525 Protein_215 transposase -
LCG89_RS14365 (LCG89_14365) 194774..195244 - 471 Protein_216 sigma 54-interacting transcriptional regulator -
LCG89_RS14370 (LCG89_14370) 195285..195933 + 649 Protein_217 IS6 family transposase -
LCG89_RS14375 (LCG89_14375) 195992..196180 - 189 Protein_218 hypothetical protein -
LCG89_RS14380 (LCG89_14380) 196181..197038 - 858 WP_002292680.1 ParA family protein -
LCG89_RS14385 (LCG89_14385) 197323..197609 - 287 Protein_220 type II toxin-antitoxin system RelE/ParE family toxin -
LCG89_RS14390 (LCG89_14390) 197599..197928 - 330 WP_002292678.1 AbrB family transcriptional regulator -
LCG89_RS14395 (LCG89_14395) 198005..198277 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
LCG89_RS14400 (LCG89_14400) 198277..198540 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LCG89_RS14405 (LCG89_14405) 198774..199682 + 909 Protein_224 IS982 family transposase -
LCG89_RS14410 (LCG89_14410) 199685..200860 - 1176 WP_002336205.1 MFS transporter -
LCG89_RS14415 (LCG89_14415) 200863..203112 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..213284 213284
- inside IScluster/Tn - - 189030..211032 22002


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T217796 WP_002314366.1 NZ_CP083913:c198277-198005 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T217796 NZ_CP114853:794230-794640 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT217796 WP_002297422.1 NZ_CP083913:c198540-198277 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT217796 NZ_CP114853:793983-794249 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References