Detailed information of TA system TA217774

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28043..28307
• Replicon: plasmid unnamed2
• Accession: NZ_CP083890
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: LCE25_RS24675
• Protein ID: WP_001303307.1
• Coordinates: 28043..28195 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	28245..28307 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS24650 (23046)	23046..25214	+	2169	WP_000698368.1	DotA/TraY family protein	-
LCE25_RS24655 (25285)	25285..25947	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
LCE25_RS24660 (26019)	26019..26228	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
LCE25_RS24665 (26620)	26620..26796	+	177	WP_001054900.1	hypothetical protein	-
- (27282)	27282..27333	+	52	NuclAT_1	-	-
- (27282)	27282..27333	+	52	NuclAT_1	-	-
- (27282)	27282..27333	+	52	NuclAT_1	-	-
- (27282)	27282..27333	+	52	NuclAT_1	-	-
LCE25_RS24670 (27720)	27720..27971	+	252	WP_001291965.1	hypothetical protein	-
LCE25_RS24675 (28043)	28043..28195	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
- (28245)	28245..28307	+	63	NuclAT_0	-	Antitoxin
- (28245)	28245..28307	+	63	NuclAT_0	-	Antitoxin
- (28245)	28245..28307	+	63	NuclAT_0	-	Antitoxin
- (28245)	28245..28307	+	63	NuclAT_0	-	Antitoxin
LCE25_RS24680 (28487)	28487..29695	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
LCE25_RS24685 (29714)	29714..30784	+	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
LCE25_RS24690 (30777)	30777..33068	+	2292	WP_001289271.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA2 / cmlA1 / ant(3'')-Ia / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..137647	137647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T217774 WP_001303307.1 NZ_CP083890:c28195-28043 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T217774 NZ_CP114786:5904326-5904946 [Roseibium sp. Sym1]
ATGACACAGAGCGACTTCCCCTCCCCGCCCGACACGATCGACCCGGCCAGACTGACCGGTATTCTCGATTTCCTCCAGGC
CTGCGAACAACTGAAGGACACGCTCCGCTCGGGGGCAACCCGGAAGGGCCGGCCGGAAAGCACCGCCGAACACAGCTGGC
GGCTGGCGCTGATGATCCTGCTGTTCGAACGTGACCTGAAGGGACTGGATGTTCCCAAGCTGCTCAAGCTCAGCCTGGTC
CACGATCTCGGCGAAGCCATCTCCGGCGATGTGCCTGCGCCCCACCAGACACCGGGGGACGATCGCGCGGCCCGCGAGCG
GCGCGACTTCCAGACCCTGTGCACGCCCTTGCCCCTGGACCTTGCAGCGGAGCTGCTGGCCCTTTTCGATGAATACAACG
CTGCGGAAAGCCTCGAAGCGCAGCTTGCCAAGGCTTTCGACAAGCTTGAGACCATGCTGCAGCACCTGCTGATGCCGACA
CGGGATGCGATTTTCTACGACTTCAATCTGGGCTACGGGCGGGACCGGACGGACTGTTCCCCGCTTACGCGGCAAATCCG
CGACAGGGTCGATGTACAGACACGGGACATCCTGATCGGGCTGGAACAGGAAACCGCCTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT217774 NZ_CP083890:28245-28307 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin