Detailed information of TA system TA217768

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148799..149225
• Replicon: plasmid unnamed1
• Accession: NZ_CP083889
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCE25_RS24150
• Protein ID: WP_096937776.1
• Coordinates: 148799..148924 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	149001..149225 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS24115 (143972)	143972..144373	-	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-
LCE25_RS24120 (144466)	144466..145155	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
LCE25_RS24125 (145342)	145342..145725	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCE25_RS24130 (146046)	146046..146648	+	603	WP_077250849.1	transglycosylase SLT domain-containing protein	-
LCE25_RS24135 (146945)	146945..147766	-	822	WP_001234487.1	DUF932 domain-containing protein	-
LCE25_RS24140 (147877)	147877..148173	-	297	WP_001272245.1	hypothetical protein	-
LCE25_RS24145 (148225)	148225..148498	+	274	Protein_157	hypothetical protein	-
LCE25_RS24150 (148799)	148799..148924	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCE25_RS24155 (148866)	148866..149015	-	150	Protein_159	plasmid maintenance protein Mok	-
- (149001)	149001..149225	-	225	NuclAT_0	-	Antitoxin
- (149001)	149001..149225	-	225	NuclAT_0	-	Antitoxin
- (149001)	149001..149225	-	225	NuclAT_0	-	Antitoxin
- (149001)	149001..149225	-	225	NuclAT_0	-	Antitoxin
LCE25_RS24160 (149194)	149194..149956	-	763	Protein_160	plasmid SOS inhibition protein A	-
LCE25_RS24165 (149953)	149953..150387	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
LCE25_RS24170 (150456)	150456..152420	-	1965	WP_224446611.1	ParB/RepB/Spo0J family partition protein	-
LCE25_RS24175 (152479)	152479..152712	-	234	WP_000006012.1	DUF905 family protein	-
LCE25_RS24180 (152770)	152770..153309	-	540	WP_001593051.1	single-stranded DNA-binding protein	-
LCE25_RS24185 (153335)	153335..153541	-	207	WP_000547969.1	hypothetical protein	-
LCE25_RS24190 (153611)	153611..153691	+	81	Protein_166	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..219996	219996
	-	flank	IS/Tn	-	-	153862..154833	971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T217768 WP_096937776.1 NZ_CP083889:c148924-148799 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T217768 NZ_CP114772:4433791-4434009 [Raoultella planticola]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGATCACCGTCTTGCTGAAT
TGACCATGAATAAACTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT217768 NZ_CP083889:c149225-149001 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin