Detailed information of TA system TA217763

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110718..110972
• Replicon: plasmid unnamed1
• Accession: NZ_CP083889
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LCE25_RS23925
• Protein ID: WP_001312851.1
• Coordinates: 110718..110867 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	110911..110972 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS23895 (107473)	107473..107631	-	159	Protein_107	type II toxin-antitoxin system antitoxin YacA	-
LCE25_RS23900 (108546)	108546..109403	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
LCE25_RS23905 (109396)	109396..109878	-	483	WP_001273588.1	hypothetical protein	-
LCE25_RS23910 (109871)	109871..109918	-	48	WP_229471593.1	hypothetical protein	-
LCE25_RS23915 (109909)	109909..110160	+	252	WP_223195197.1	replication protein RepA	-
LCE25_RS23920 (110177)	110177..110434	-	258	WP_000083845.1	replication regulatory protein RepA	-
LCE25_RS23925 (110718)	110718..110867	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (110911)	110911..110972	+	62	NuclAT_1	-	Antitoxin
- (110911)	110911..110972	+	62	NuclAT_1	-	Antitoxin
- (110911)	110911..110972	+	62	NuclAT_1	-	Antitoxin
- (110911)	110911..110972	+	62	NuclAT_1	-	Antitoxin
LCE25_RS23930 (111228)	111228..111302	-	75	Protein_114	endonuclease	-
LCE25_RS23935 (111548)	111548..111760	-	213	WP_001299730.1	ANR family transcriptional regulator	-
LCE25_RS23940 (111896)	111896..112456	-	561	WP_000139315.1	fertility inhibition protein FinO	-
LCE25_RS23945 (112559)	112559..113419	-	861	WP_000704513.1	alpha/beta hydrolase	-
LCE25_RS23950 (113478)	113478..114224	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..219996	219996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T217763 WP_001312851.1 NZ_CP083889:c110867-110718 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T217763 NZ_CP114771:c5509848-5509537 [Endozoicomonas sp. GU-1]
ATGATTTTCATAGAAACCAGCCGCTTTACCAAATTGCTATCCGGATATTTATCAGATGATGAATACCGGGTATTGCAGTG
GCACCTTCAACAGAAACCGGATTCTGGCGATCTTATCAGGGGTAGTGGGGGTGTCCGTAAAATACGATGGGCACCGGATG
GAAAGGGTAAAAGTGGTAGTATCCGGGTTATTTATTACTGGAAAAAGAGTGATTTTGAAATCTGGATGTTGACCTTATAT
CGCAAGTCGGAGCGTGAAAGTATTCCTGGTCATATCCTGAAAAAGGTAGCGGAGGCTATAAACGATGAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT217763 NZ_CP083889:110911-110972 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin