Detailed information of TA system TA217747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1091638..1092365
• Replicon: chromosome
• Accession: NZ_CP083888
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LCE25_RS05305
• Protein ID: WP_000547564.1
• Coordinates: 1091638..1091949 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCE25_RS05310
• Protein ID: WP_000126294.1
• Coordinates: 1091946..1092365 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS05275 (1086780)	1086780..1088489	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LCE25_RS05280 (1088499)	1088499..1089041	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LCE25_RS05285 (1089041)	1089041..1089808	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LCE25_RS05290 (1089805)	1089805..1090215	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LCE25_RS05295 (1090208)	1090208..1090678	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LCE25_RS05300 (1090703)	1090703..1091464	+	762	WP_001026446.1	hypothetical protein	-
LCE25_RS05305 (1091638)	1091638..1091949	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LCE25_RS05310 (1091946)	1091946..1092365	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LCE25_RS05315 (1092479)	1092479..1093903	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
LCE25_RS05320 (1093912)	1093912..1095369	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LCE25_RS05325 (1095629)	1095629..1096639	+	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
LCE25_RS05330 (1096788)	1096788..1097315	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T217747 WP_000547564.1 NZ_CP083888:1091638-1091949 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T217747 NZ_CP114768:c102762-102502 [Hymenobacter canadensis]
ATGAACACGCGCCTGATTCGGGTGGGCAATTCCCAGGGGATTGTGCTGCCTAAGAAGTTACTGCAGCAGTACAATCTCAC
CGGCGAGGTAGACCTGCAGCCCACGCCCGAAGGCCTGCTCATTAAGCCGGTCGTGAAGACCCGCCGCCAGGGCTGGGACG
AGCGCTTTCAACAAGCCATTGCCCAGGGCCAGGCTCCGGAGGGCGAACTGTTGGAGGGCTTTTCGGATGAGGCCTTTGAA
GAAACGGAATGGCAGTGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT217747 WP_000126294.1 NZ_CP083888:1091946-1092365 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT217747 NZ_CP114768:c102514-102182 [Hymenobacter canadensis]
ATGGCAGTGGTAGGCGTGCAGCGCTTCGAGGTGTGGCTCATCAACCTTGACCCGACCCAGGGCAGCGAAATCAATAAGAC
CCGCCCCTGTGTCGTGCTCTCCCCCGACGAGATGAACCGCTACCTGCGCACGGTCACTATTGCGGCTCTGACGAGCACCC
GGCGTGACTACCCTTCCCGGGTGGACTGCACTTTCCAGGGCAAAGAGGGCCAGGTCGCGCTCGACCACATCCGCTCCGTG
GACAAAACCCGCCTGGTACGCCGCTTAGGTGTATTGCCCAAAGCCACGGCGCAGGCCGTCTGTGACCGGCTGCAGGAGAT
ATTCCAGTACTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5