Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 601370..602169 Replicon chromosome
Accession NZ_CP083888
Organism Escherichia coli strain NDM1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LCE25_RS02930 Protein ID WP_000347251.1
Coordinates 601370..601834 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LCE25_RS02935 Protein ID WP_001307405.1
Coordinates 601834..602169 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE25_RS02900 (596371) 596371..596805 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LCE25_RS02905 (596823) 596823..597701 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LCE25_RS02910 (597691) 597691..598470 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LCE25_RS02915 (598481) 598481..598954 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LCE25_RS02920 (598977) 598977..600257 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
LCE25_RS02925 (600506) 600506..601315 + 810 WP_000072192.1 aga operon transcriptional regulator AgaR -
LCE25_RS02930 (601370) 601370..601834 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LCE25_RS02935 (601834) 601834..602169 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LCE25_RS02940 (602318) 602318..603889 - 1572 WP_001273761.1 galactarate dehydratase -
LCE25_RS02945 (604264) 604264..605598 + 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
LCE25_RS02950 (605614) 605614..606384 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T217743 WP_000347251.1 NZ_CP083888:c601834-601370 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T217743 NZ_CP114761:c5058231-5057866 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT217743 WP_001307405.1 NZ_CP083888:c602169-601834 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT217743 NZ_CP114761:c5057873-5057550 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References