Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3684422..3684963 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LCE23_RS18810 Protein ID WP_000615983.1
Coordinates 3684685..3684963 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LCE23_RS18805 Protein ID WP_000729703.1
Coordinates 3684422..3684682 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS18785 (3680097) 3680097..3680882 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LCE23_RS18790 (3680854) 3680854..3682566 + 1713 Protein_3488 flagellar biosynthesis protein FlhA -
LCE23_RS18795 (3682790) 3682790..3683287 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LCE23_RS18800 (3683463) 3683463..3684212 - 750 WP_000056849.1 C40 family peptidase -
LCE23_RS18805 (3684422) 3684422..3684682 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LCE23_RS18810 (3684685) 3684685..3684963 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LCE23_RS18815 (3685119) 3685119..3685859 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LCE23_RS18820 (3685830) 3685830..3686597 - 768 WP_000333380.1 class II glutamine amidotransferase -
LCE23_RS18825 (3686803) 3686803..3687381 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA / rhs/PAAR / vgrG/tssI 3593144..3699932 106788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T217740 WP_000615983.1 NZ_CP083886:3684685-3684963 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T217740 NZ_CP114761:2592852-2593133 [Pseudomonas aeruginosa]
ATGACCTATGAGCTGGAGTTCCTGCCCTCAGCCCTGAAGGAGTGGCAGAAGCTCGGCCATACGGTGCGCGAGCAGATCAA
AAAGAAACTGCGCGAGCGCCTGGATAATCCCAAGGTTCAGGCCGACGCCCTCAGGGATATGCCGGGGCACTACAAAATCA
AGCTGCGTGCATCGGGCTATCGTCTGGTATACCGCGTCGAAGATGAGCGCGTGGTGGTCGTGGTGGTTGCCGTTGGCAAG
CGCGAGCGTGGGGCCGCTTACCAATCTGCCAAGGGCCGTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT217740 WP_000729703.1 NZ_CP083886:3684422-3684682 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT217740 NZ_CP114761:2592617-2592862 [Pseudomonas aeruginosa]
ATGCAGAGTATCTTGGCTGACGTCGCTGTCAGCGTTTCTGAGTTGAAGAAAAATCCGTCGGGCATCCTGGCTGGTGCAGG
TGGTATGCCTGTCGCCGTGCTCAACCACAATCGCGTCATGGGATACATGGTGCCGGCAGAGCTCTATGAGCAGATGATGG
AGCGCCTAGATGATCTGGAGTTGGTCGAAATAGTGAAGGCTCGCTCGGGCGAGGAGTCTGTCCCGGTGCGTTTGGATGAC
CTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References