Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3678225..3678919 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LCE23_RS18770 Protein ID WP_001263489.1
Coordinates 3678225..3678623 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LCE23_RS18775 Protein ID WP_000554758.1
Coordinates 3678626..3678919 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3673813) 3673813..3673893 - 81 NuclAT_8 - -
- (3673813) 3673813..3673893 - 81 NuclAT_8 - -
- (3673813) 3673813..3673893 - 81 NuclAT_8 - -
- (3673813) 3673813..3673893 - 81 NuclAT_8 - -
LCE23_RS18745 (3674489) 3674489..3674947 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LCE23_RS18750 (3675208) 3675208..3676665 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LCE23_RS18755 (3676722) 3676722..3677243 - 522 Protein_3481 peptide chain release factor H -
LCE23_RS18760 (3677239) 3677239..3677445 - 207 Protein_3482 RtcB family protein -
LCE23_RS18765 (3677763) 3677763..3678215 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LCE23_RS18770 (3678225) 3678225..3678623 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LCE23_RS18775 (3678626) 3678626..3678919 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LCE23_RS18780 (3678971) 3678971..3680026 - 1056 WP_001226164.1 DNA polymerase IV -
LCE23_RS18785 (3680097) 3680097..3680882 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LCE23_RS18790 (3680854) 3680854..3682566 + 1713 Protein_3488 flagellar biosynthesis protein FlhA -
LCE23_RS18795 (3682790) 3682790..3683287 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA / rhs/PAAR / vgrG/tssI 3593144..3699932 106788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T217739 WP_001263489.1 NZ_CP083886:c3678623-3678225 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T217739 NZ_CP114761:2008394-2008981 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGTGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT217739 WP_000554758.1 NZ_CP083886:c3678919-3678626 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT217739 NZ_CP114761:2009048-2009902 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCT
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References