Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1863265..1863767 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag LCE23_RS09835 Protein ID WP_000767831.1
Coordinates 1863513..1863767 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LCE23_RS09830 Protein ID WP_001259255.1
Coordinates 1863265..1863516 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS09805 (1858442) 1858442..1859509 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
LCE23_RS09810 (1859509) 1859509..1860579 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
LCE23_RS09815 (1860576) 1860576..1861880 - 1305 WP_001446927.1 histidinol dehydrogenase -
LCE23_RS09820 (1861886) 1861886..1862785 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
LCE23_RS09825 (1862931) 1862931..1862981 - 51 WP_001364200.1 his operon leader peptide -
LCE23_RS09830 (1863265) 1863265..1863516 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LCE23_RS09835 (1863513) 1863513..1863767 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LCE23_RS09840 (1863850) 1863850..1864674 + 825 WP_000754737.1 SDR family oxidoreductase -
LCE23_RS09845 (1864720) 1864720..1865649 + 930 WP_024195433.1 LysR substrate-binding domain-containing protein -
LCE23_RS09850 (1865864) 1865864..1865926 + 63 WP_010723108.1 membrane protein YoeI -
LCE23_RS09855 (1865916) 1865916..1867274 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LCE23_RS09860 (1867453) 1867453..1868511 + 1059 WP_000492321.1 thiosulfate utilization transporter TsuA/YeeE -
LCE23_RS09865 (1868525) 1868525..1868752 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T217727 WP_000767831.1 NZ_CP083886:1863513-1863767 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T217727 NZ_CP114600:429535-429669 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT217727 WP_001259255.1 NZ_CP083886:1863265-1863516 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT217727 NZ_CP114600:429319-429471 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References