Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 716866..717559 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name mqsR Uniprot ID U9ZN09
Locus tag LCE23_RS04450 Protein ID WP_000415585.1
Coordinates 716866..717162 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag LCE23_RS04455 Protein ID WP_000650107.1
Coordinates 717164..717559 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS04415 (712000) 712000..712479 + 480 WP_000065332.1 Hcp family type VI secretion system effector -
LCE23_RS04420 (712482) 712482..713192 + 711 WP_000834030.1 hypothetical protein -
LCE23_RS04425 (713199) 713199..713531 + 333 WP_000914690.1 DUF2645 family protein -
LCE23_RS04430 (713577) 713577..714926 - 1350 WP_000673358.1 quorum sensing histidine kinase QseC -
LCE23_RS04435 (714923) 714923..715582 - 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB -
LCE23_RS04440 (715734) 715734..716126 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
LCE23_RS04445 (716179) 716179..716661 + 483 WP_000183505.1 GyrI-like domain-containing protein -
LCE23_RS04450 (716866) 716866..717162 + 297 WP_000415585.1 type II toxin-antitoxin system toxin MqsR Toxin
LCE23_RS04455 (717164) 717164..717559 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
LCE23_RS04460 (717692) 717692..719299 + 1608 WP_001375094.1 ABC transporter substrate-binding protein -
LCE23_RS04465 (719437) 719437..721695 + 2259 WP_001281841.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(2-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11261.99 Da        Isoelectric Point: 8.9070

>T217723 WP_000415585.1 NZ_CP083886:716866-717162 [Escherichia coli]
MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T217723 NZ_CP114594:19103-19390 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTAAAAAAATGCAGTGAAAATCCTCATATAGCCTCTGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT217723 WP_000650107.1 NZ_CP083886:717164-717559 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT217723 NZ_CP114594:18855-19106 [Escherichia coli]
ATGCCCAGTATTATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGGATGC
TTGATGCCCTGGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZN09

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References