Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 651161..651888 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LCE23_RS04135 Protein ID WP_000550189.1
Coordinates 651161..651475 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LCE23_RS04140 Protein ID WP_000560266.1
Coordinates 651472..651888 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS04115 (647328) 647328..648314 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
LCE23_RS04120 (648393) 648393..649076 - 684 WP_001183043.1 vancomycin high temperature exclusion protein -
LCE23_RS04125 (649153) 649153..649656 - 504 WP_001295542.1 M48 family metallopeptidase -
LCE23_RS04130 (649741) 649741..650877 + 1137 WP_000018681.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LCE23_RS04135 (651161) 651161..651475 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LCE23_RS04140 (651472) 651472..651888 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LCE23_RS04145 (651933) 651933..653951 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LCE23_RS04150 (654377) 654377..656728 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T217722 WP_000550189.1 NZ_CP083886:651161-651475 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T217722 NZ_CP114593:c50438-50178 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGC
GAGGGAAAAAATGCTAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT217722 WP_000560266.1 NZ_CP083886:651472-651888 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT217722 NZ_CP114593:c50188-49937 [Escherichia coli]
ATGCTAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTCCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References