Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 607824..608623 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag LCE23_RS03910 Protein ID WP_000347275.1
Coordinates 607824..608288 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LCE23_RS03915 Protein ID WP_001307405.1
Coordinates 608288..608623 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS03880 (602825) 602825..603259 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LCE23_RS03885 (603277) 603277..604155 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LCE23_RS03890 (604145) 604145..604924 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LCE23_RS03895 (604935) 604935..605408 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LCE23_RS03900 (605431) 605431..606711 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LCE23_RS03905 (606960) 606960..607769 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LCE23_RS03910 (607824) 607824..608288 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LCE23_RS03915 (608288) 608288..608623 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LCE23_RS03920 (608772) 608772..610343 - 1572 WP_001273752.1 galactarate dehydratase -
LCE23_RS03925 (610718) 610718..612052 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LCE23_RS03930 (612068) 612068..612838 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T217721 WP_000347275.1 NZ_CP083886:c608288-607824 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T217721 NZ_CP114593:c48645-48397 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT217721 WP_001307405.1 NZ_CP083886:c608623-608288 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT217721 NZ_CP114593:c48400-48053 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References