Detailed information of TA system TA217715

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41539..41793
• Replicon: plasmid unnamed1
• Accession: NZ_CP083884
• Organism: Escherichia coli strain NDM2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LCE23_RS00255
• Protein ID: WP_001312851.1
• Coordinates: 41644..41793 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41539..41600 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE23_RS00230 (38287)	38287..39033	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
LCE23_RS00235 (39092)	39092..39952	+	861	WP_000704513.1	alpha/beta hydrolase	-
LCE23_RS00240 (40055)	40055..40615	+	561	WP_000139315.1	fertility inhibition protein FinO	-
LCE23_RS00245 (40751)	40751..40963	+	213	WP_001299730.1	ANR family transcriptional regulator	-
LCE23_RS00250 (41209)	41209..41283	+	75	Protein_49	endonuclease	-
- (41539)	41539..41600	-	62	NuclAT_1	-	Antitoxin
- (41539)	41539..41600	-	62	NuclAT_1	-	Antitoxin
- (41539)	41539..41600	-	62	NuclAT_1	-	Antitoxin
- (41539)	41539..41600	-	62	NuclAT_1	-	Antitoxin
LCE23_RS00255 (41644)	41644..41793	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LCE23_RS00260 (42077)	42077..42334	+	258	WP_000083845.1	replication regulatory protein RepA	-
LCE23_RS00265 (42351)	42351..42602	-	252	WP_223195197.1	replication protein RepA	-
LCE23_RS00270 (42593)	42593..42640	+	48	WP_229471593.1	hypothetical protein	-
LCE23_RS00275 (42633)	42633..43115	+	483	WP_001273588.1	hypothetical protein	-
LCE23_RS00280 (43108)	43108..43965	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
LCE23_RS00285 (44904)	44904..45557	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LCE23_RS00290 (45650)	45650..45907	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LCE23_RS00295 (45840)	45840..46241	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iucA / iucB / iucC / iucD / iutA	1..164740	164740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T217715 WP_001312851.1 NZ_CP083884:41644-41793 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T217715 NZ_CP114592:3553885-3554427 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT217715 NZ_CP083884:c41600-41539 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin