Detailed information of TA system TA217711

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3443..3712
• Replicon: plasmid unnamed1
• Accession: NZ_CP083884
• Organism: Escherichia coli strain NDM2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCE23_RS00030
• Protein ID: WP_001375150.1
• Coordinates: 3596..3712 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	3443..3508 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE23_RS00010	91..2055	+	1965	WP_224446720.1	ParB/RepB/Spo0J family partition protein	-
LCE23_RS00015	2124..2558	+	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
LCE23_RS00020	2555..3317	+	763	Protein_3	plasmid SOS inhibition protein A	-
-	3286..3510	+	225	NuclAT_0	-	-
-	3286..3510	+	225	NuclAT_0	-	-
-	3286..3510	+	225	NuclAT_0	-	-
-	3286..3510	+	225	NuclAT_0	-	-
-	3443..3508	-	66	-	-	Antitoxin
LCE23_RS00025	3496..3645	+	150	Protein_4	plasmid maintenance protein Mok	-
LCE23_RS00030	3596..3712	+	117	WP_001375150.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCE23_RS00035	4013..4286	-	274	Protein_6	hypothetical protein	-
LCE23_RS00040	4338..4634	+	297	WP_001272245.1	hypothetical protein	-
LCE23_RS00045	4745..5566	+	822	WP_001234487.1	DUF932 domain-containing protein	-
LCE23_RS00050	5863..6465	-	603	WP_077250849.1	transglycosylase SLT domain-containing protein	-
LCE23_RS00055	6786..7169	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCE23_RS00060	7356..8045	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
LCE23_RS00065	8138..8539	+	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iucA / iucB / iucC / iucD / iutA	1..164740	164740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4481.27 Da
• Isoelectric Point: 8.4890

>T217711 WP_001375150.1 NZ_CP083884:3596-3712 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T217711 NZ_CP114592:2725438-2725545 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT217711 NZ_CP083884:c3508-3443 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin