Detailed information of TA system TA217707

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75965..76386
• Replicon: plasmid unnamed1
• Accession: NZ_CP083881
• Organism: Escherichia coli strain NDM3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCE26_RS23310
• Protein ID: WP_001375150.1
• Coordinates: 76270..76386 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	75965..76163 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE26_RS23270 (71661)	71661..73055	+	1395	WP_001037799.1	carbohydrate porin	-
LCE26_RS23275 (73301)	73301..73405	-	105	Protein_83	thiosulfate reductase cytochrome B subunit	-
LCE26_RS23280 (73462)	73462..74155	+	694	Protein_84	IS1 family transposase	-
LCE26_RS23285 (74282)	74282..74794	+	513	WP_223350513.1	potassium channel family protein	-
LCE26_RS23290 (74879)	74879..75130	+	252	Protein_86	iron-containing alcohol dehydrogenase	-
LCE26_RS23295 (75419)	75419..75736	-	318	Protein_87	IS110 family transposase	-
LCE26_RS23300 (75891)	75891..75996	+	106	Protein_88	plasmid SOS inhibition protein A	-
- (75965)	75965..76163	+	199	NuclAT_0	-	Antitoxin
- (75965)	75965..76163	+	199	NuclAT_0	-	Antitoxin
- (75965)	75965..76163	+	199	NuclAT_0	-	Antitoxin
- (75965)	75965..76163	+	199	NuclAT_0	-	Antitoxin
LCE26_RS23305 (76170)	76170..76319	+	150	Protein_89	DUF5431 family protein	-
LCE26_RS23310 (76270)	76270..76386	+	117	WP_001375150.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCE26_RS23315 (76687)	76687..76984	-	298	Protein_91	hypothetical protein	-
LCE26_RS23320 (77054)	77054..77260	+	207	WP_000275854.1	hypothetical protein	-
LCE26_RS23325 (77285)	77285..77572	+	288	WP_000107242.1	hypothetical protein	-
LCE26_RS23330 (77691)	77691..78512	+	822	WP_001234465.1	DUF932 domain-containing protein	-
LCE26_RS23335 (78808)	78808..79380	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
LCE26_RS23340 (79750)	79750..80133	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCE26_RS23345 (80324)	80324..80971	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
LCE26_RS23350 (81107)	81107..81322	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..159955	159955
	-	flank	IS/Tn	-	-	73778..74155	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4481.27 Da
• Isoelectric Point: 8.4890

>T217707 WP_001375150.1 NZ_CP083881:76270-76386 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T217707 NZ_CP114592:1831168-1831422 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT217707 NZ_CP083881:75965-76163 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin