Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3698391..3699085 | Replicon | chromosome |
| Accession | NZ_CP083880 | ||
| Organism | Escherichia coli strain NDM3 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | LCE26_RS17945 | Protein ID | WP_001263489.1 |
| Coordinates | 3698391..3698789 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | LCE26_RS17950 | Protein ID | WP_000554758.1 |
| Coordinates | 3698792..3699085 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3693979) | 3693979..3694059 | - | 81 | NuclAT_13 | - | - |
| - (3693979) | 3693979..3694059 | - | 81 | NuclAT_13 | - | - |
| - (3693979) | 3693979..3694059 | - | 81 | NuclAT_13 | - | - |
| - (3693979) | 3693979..3694059 | - | 81 | NuclAT_13 | - | - |
| LCE26_RS17920 (3694655) | 3694655..3695113 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LCE26_RS17925 (3695374) | 3695374..3696831 | + | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| LCE26_RS17930 (3696888) | 3696888..3697409 | - | 522 | Protein_3504 | peptide chain release factor H | - |
| LCE26_RS17935 (3697405) | 3697405..3697611 | - | 207 | Protein_3505 | RtcB family protein | - |
| LCE26_RS17940 (3697929) | 3697929..3698381 | - | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| LCE26_RS17945 (3698391) | 3698391..3698789 | - | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LCE26_RS17950 (3698792) | 3698792..3699085 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LCE26_RS17955 (3699137) | 3699137..3700192 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| LCE26_RS17960 (3700263) | 3700263..3701048 | - | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| LCE26_RS17965 (3701020) | 3701020..3702732 | + | 1713 | Protein_3511 | flagellar biosynthesis protein FlhA | - |
| LCE26_RS17970 (3702956) | 3702956..3703453 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3641582..3704255 | 62673 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T217700 WP_001263489.1 NZ_CP083880:c3698789-3698391 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T217700 NZ_CP114592:292549-293076 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGAATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGAATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT217700 WP_000554758.1 NZ_CP083880:c3699085-3698792 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT217700 NZ_CP114592:292276-292542 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A090J8B1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |