Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 647138..647937 Replicon chromosome
Accession NZ_CP083880
Organism Escherichia coli strain NDM3

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag LCE26_RS03155 Protein ID WP_000347272.1
Coordinates 647138..647602 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LCE26_RS03160 Protein ID WP_001307405.1
Coordinates 647602..647937 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE26_RS03125 (642139) 642139..642573 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LCE26_RS03130 (642591) 642591..643469 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
LCE26_RS03135 (643459) 643459..644238 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LCE26_RS03140 (644249) 644249..644722 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LCE26_RS03145 (644745) 644745..646025 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
LCE26_RS03150 (646274) 646274..647083 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
LCE26_RS03155 (647138) 647138..647602 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LCE26_RS03160 (647602) 647602..647937 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LCE26_RS03165 (648086) 648086..649657 - 1572 WP_001273738.1 galactarate dehydratase -
LCE26_RS03170 (650032) 650032..651366 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LCE26_RS03175 (651382) 651382..652152 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 647138..658590 11452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T217684 WP_000347272.1 NZ_CP083880:c647602-647138 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T217684 NZ_CP114578:c2727159-2726302 [Legionella pneumophila]
ATGACAAAAGATATAAGTGCTTCCATTCGAGCACGACTTAAAAAAATAGCAAAAGCTGAAGGATCAAAGTCAGATTTTAT
TGCTGTCTTAACCCGTTATGGTCTCGAGCGACTGTTATACCGAATTGGCATATCTGAATACGCCAGTCAATTTTTACTAA
AAGGCGCTTTACTATTTAATCTTTGGTATGACATGCCGCACCGCCCAACCAAGGATATTGATTTATTAGGGTTTGGTGAT
AATGATCTGGCTTATATCCAGAAAACTTTCAGGGAAATTTGTAGTCTTTCAGCGGACGATGGGATGAATTTTCTTTCTAA
ATCTGTTGAAGTAAGCACTATCAAAAAAGATGGTGGTTATACTGGTGCAAGGGTTGAGTTATTTGGAGAGTTGGCAAAAG
CAAAGATCAAAATACAAGTTGATATTGGTTATGGAGATGCAGTAACTCCTGGCCCAATAGACGCTAATTACCCTGTACTT
TTAAGCGATCTTCCTGCTCCACAAATTCGTACTTATCCCGTTTATACTGTTATCGCAGAAAAATTGCACGCAATTGCATT
ATTGGGTATGGCTAATAGTCGATTGAAAGATTACTTAGACCTTTATGTGTTATTAACTCAAGAACAATTGGATAATCAAG
TTTTAGCCCAAGCTATACATGCCACATTTATTCGTCGGGGCATGACTGTACCTGAAGTACTACCTGTAGGTCTGTCCGAT
GAATTCGCCAATGACCCAACTAGAATATCAATTTGGAACGCTTTTTTAAGCAAAAATGAATTGGAACAGAGATCATTAAC
AGAAGTTATAGAGGTGATCATAAGTTTAATTCAAACACCATTTTCTTTGGCAAGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT217684 WP_001307405.1 NZ_CP083880:c647937-647602 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT217684 NZ_CP114578:c2727764-2727156 [Legionella pneumophila]
ATGAATACTAAAAACACCCATAGTCAAAAGGCTCTGAATCTTGCTAAAGAAAAAGGAATCGTACGTTCCTCTGAGTTTGT
AGAAGAAGGCATTCCAAGAATCGTCATATCTCGTCTAGTTGCAAATCAACAATTGCAACAGATTGAGAGGGGGCTTTATT
GTCTTCCTGAAAAGGAATTCTCTGAAAAGGAGAGTTTGTTGGTTATTGCCACTCGAGTGCCACAAGCCATTTTTTGTCTT
TTTACAGCACTTCAAGTTCATGGACTTACTACACAATTGCCAAGAAAGATTTGGATAGCCATGCCTATTGATAGTCGTGT
ACCTAAAATAGATTATCCACCTATAAAAATGGTGAAGTATTCAGGAGAAGCTTATTCTCAGGGGATTGAAACGCAAGAGG
CTGATGGTGTTTCATTTCGTGTATATAATGTTGCTAAAACAATTGCTGATTGCTTTAAGCATAGGAATAAAATTGGTATG
GATGTAGCAATTGAAGCTTTGAAAGATGCCTATGCCCAAAATAAAGTGACTGCTGATGAACTATGGCATTATGCGAAAAT
TTGTCGTGTTGCAAAAGTAATGCGTCCTTATCTAGAGGCTATCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References