Detailed information of TA system TA217678

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26662..27083
• Replicon: plasmid unnamed1
• Accession: NZ_CP083878
• Organism: Escherichia coli strain NDM4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LCE22_RS23010
• Protein ID: WP_001375150.1
• Coordinates: 26967..27083 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	26662..26860 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE22_RS22970 (22358)	22358..23752	+	1395	WP_001037799.1	carbohydrate porin	-
LCE22_RS22975 (23998)	23998..24102	-	105	Protein_24	thiosulfate reductase cytochrome B subunit	-
LCE22_RS22980 (24159)	24159..24852	+	694	Protein_25	IS1 family transposase	-
LCE22_RS22985 (24979)	24979..25491	+	513	WP_223350513.1	potassium channel family protein	-
LCE22_RS22990 (25576)	25576..25827	+	252	Protein_27	iron-containing alcohol dehydrogenase	-
LCE22_RS22995 (26116)	26116..26433	-	318	Protein_28	IS110 family transposase	-
LCE22_RS23000 (26588)	26588..26693	+	106	Protein_29	plasmid SOS inhibition protein A	-
- (26662)	26662..26860	+	199	NuclAT_0	-	Antitoxin
- (26662)	26662..26860	+	199	NuclAT_0	-	Antitoxin
- (26662)	26662..26860	+	199	NuclAT_0	-	Antitoxin
- (26662)	26662..26860	+	199	NuclAT_0	-	Antitoxin
LCE22_RS23005 (26867)	26867..27016	+	150	Protein_30	DUF5431 family protein	-
LCE22_RS23010 (26967)	26967..27083	+	117	WP_001375150.1	type I toxin-antitoxin system Hok family toxin	Toxin
LCE22_RS23015 (27384)	27384..27681	-	298	Protein_32	hypothetical protein	-
LCE22_RS23020 (27751)	27751..27957	+	207	WP_000275854.1	hypothetical protein	-
LCE22_RS23025 (27982)	27982..28269	+	288	WP_000107242.1	hypothetical protein	-
LCE22_RS23030 (28388)	28388..29209	+	822	WP_001234465.1	DUF932 domain-containing protein	-
LCE22_RS23035 (29505)	29505..30077	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
LCE22_RS23040 (30447)	30447..30830	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LCE22_RS23045 (31021)	31021..31668	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
LCE22_RS23050 (31804)	31804..32019	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..158041	158041
	-	flank	IS/Tn	-	-	26116..26484	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4481.27 Da
• Isoelectric Point: 8.4890

>T217678 WP_001375150.1 NZ_CP083878:26967-27083 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T217678 NZ_CP114573:c35666-35250 [Enterobacter hormaechei]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT217678 NZ_CP083878:26662-26860 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin