217674

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3704527..3705068 Replicon chromosome
Accession NZ_CP083877
Organism Escherichia coli strain NDM4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LCE22_RS17980 Protein ID WP_000615983.1
Coordinates 3704790..3705068 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LCE22_RS17975 Protein ID WP_000729703.1
Coordinates 3704527..3704787 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE22_RS17955 (3700202) 3700202..3700987 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LCE22_RS17960 (3700959) 3700959..3702671 + 1713 Protein_3510 flagellar biosynthesis protein FlhA -
LCE22_RS17965 (3702895) 3702895..3703392 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LCE22_RS17970 (3703568) 3703568..3704317 - 750 WP_000056849.1 C40 family peptidase -
LCE22_RS17975 (3704527) 3704527..3704787 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LCE22_RS17980 (3704790) 3704790..3705068 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LCE22_RS17985 (3705224) 3705224..3705964 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LCE22_RS17990 (3705935) 3705935..3706702 - 768 WP_000333380.1 class II glutamine amidotransferase -
LCE22_RS17995 (3706908) 3706908..3707486 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T217674 WP_000615983.1 NZ_CP083877:3704790-3705068 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T217674 NZ_CP114571:c3021568-3021215 [Enterobacter hormaechei]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT217674 WP_000729703.1 NZ_CP083877:3704527-3704787 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT217674 NZ_CP114571:c3021210-3020908 [Enterobacter hormaechei]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References