Detailed information of TA system TA217673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3698330..3699024
• Replicon: chromosome
• Accession: NZ_CP083877
• Organism: Escherichia coli strain NDM4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LCE22_RS17940
• Protein ID: WP_001263489.1
• Coordinates: 3698330..3698728 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LCE22_RS17945
• Protein ID: WP_000554758.1
• Coordinates: 3698731..3699024 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3693918)	3693918..3693998	-	81	NuclAT_13	-	-
- (3693918)	3693918..3693998	-	81	NuclAT_13	-	-
- (3693918)	3693918..3693998	-	81	NuclAT_13	-	-
- (3693918)	3693918..3693998	-	81	NuclAT_13	-	-
LCE22_RS17915 (3694594)	3694594..3695052	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LCE22_RS17920 (3695313)	3695313..3696770	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LCE22_RS17925 (3696827)	3696827..3697348	-	522	Protein_3503	peptide chain release factor H	-
LCE22_RS17930 (3697344)	3697344..3697550	-	207	Protein_3504	RtcB family protein	-
LCE22_RS17935 (3697868)	3697868..3698320	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LCE22_RS17940 (3698330)	3698330..3698728	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LCE22_RS17945 (3698731)	3698731..3699024	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LCE22_RS17950 (3699076)	3699076..3700131	-	1056	WP_001226164.1	DNA polymerase IV	-
LCE22_RS17955 (3700202)	3700202..3700987	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LCE22_RS17960 (3700959)	3700959..3702671	+	1713	Protein_3510	flagellar biosynthesis protein FlhA	-
LCE22_RS17965 (3702895)	3702895..3703392	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3641521..3704194	62673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T217673 WP_001263489.1 NZ_CP083877:c3698728-3698330 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T217673 NZ_CP114571:2864716-2864895 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT217673 WP_000554758.1 NZ_CP083877:c3699024-3698731 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT217673 NZ_CP114571:2864917-2865321 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGAAGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCCGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAGTACGCCGGTGCGGTATGGGCCCTAGTTGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTACCCTCATTACTGATCCGACGTATTGATCA
GTTTGTGGCCGCGCATCCTGAATATGGAAGCCGTTCGGGCTTCCTTTCTCGCGTCGCTGCTGACAAGGTTATTAATCAAC
GATAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3