Detailed information of TA system TA217661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1809975..1810477
• Replicon: chromosome
• Accession: NZ_CP083877
• Organism: Escherichia coli strain NDM4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LCE22_RS08635
• Protein ID: WP_000767832.1
• Coordinates: 1810223..1810477 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LCE22_RS08630
• Protein ID: WP_001259255.1
• Coordinates: 1809975..1810226 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE22_RS08605 (1805153)	1805153..1806220	-	1068	WP_000080064.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
LCE22_RS08610 (1806220)	1806220..1807290	-	1071	WP_094336856.1	histidinol-phosphate transaminase	-
LCE22_RS08615 (1807287)	1807287..1808591	-	1305	WP_024198577.1	histidinol dehydrogenase	-
LCE22_RS08620 (1808597)	1808597..1809496	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LCE22_RS08625 (1809642)	1809642..1809692	-	51	WP_001364200.1	his operon leader peptide	-
LCE22_RS08630 (1809975)	1809975..1810226	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LCE22_RS08635 (1810223)	1810223..1810477	+	255	WP_000767832.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LCE22_RS08640 (1810560)	1810560..1811384	+	825	WP_000754737.1	SDR family oxidoreductase	-
LCE22_RS08645 (1811430)	1811430..1812359	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
LCE22_RS08650 (1812573)	1812573..1812635	+	63	WP_097433929.1	membrane protein YoeI	-
LCE22_RS08655 (1812625)	1812625..1813983	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10232.66 Da
• Isoelectric Point: 8.0280

>T217661 WP_000767832.1 NZ_CP083877:1810223-1810477 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHYLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T217661 NZ_CP114551:c1221560-1221255 [Xylella fastidiosa subsp. pauca]
ATGAATAATGAGACGTTTAGTCGATACGACACCGCTGACTACCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGTATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAGGCGCTTGGTTTACGGATGTCATTCACTCCTTCCTCTATGCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT217661 WP_001259255.1 NZ_CP083877:1809975-1810226 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT217661 NZ_CP114551:c1221859-1221557 [Xylella fastidiosa subsp. pauca]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTTGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTGCTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LNR0