Detailed information of TA system TA217658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 753749..754442
• Replicon: chromosome
• Accession: NZ_CP083877
• Organism: Escherichia coli strain NDM4

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LCE22_RS03675
• Protein ID: WP_000415584.1
• Coordinates: 753749..754045 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LCE22_RS03680
• Protein ID: WP_000650107.1
• Coordinates: 754047..754442 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE22_RS03640 (748837)	748837..749151	-	315	WP_000958598.1	putative quinol monooxygenase	-
LCE22_RS03645 (749182)	749182..749763	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LCE22_RS03650 (750082)	750082..750414	+	333	WP_023149630.1	DUF2645 family protein	-
LCE22_RS03655 (750460)	750460..751809	-	1350	WP_224434072.1	quorum sensing histidine kinase QseC	-
LCE22_RS03660 (751806)	751806..752465	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
LCE22_RS03665 (752617)	752617..753009	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LCE22_RS03670 (753062)	753062..753544	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LCE22_RS03675 (753749)	753749..754045	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LCE22_RS03680 (754047)	754047..754442	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LCE22_RS03685 (754574)	754574..756181	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
LCE22_RS03690 (756319)	756319..758577	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T217658 WP_000415584.1 NZ_CP083877:753749-754045 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T217658 NZ_CP114551:1192606-1193217 [Xylella fastidiosa subsp. pauca]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGTGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGCCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCAATTTTTGTGTGGCCTTGATGCTGAGGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACAGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGCGGATTGGC
TAGTATCATCCGCGATGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT217658 WP_000650107.1 NZ_CP083877:754047-754442 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT217658 NZ_CP114551:1192421-1192609 [Xylella fastidiosa subsp. pauca]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATGGCATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65